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Cell No. : Cell Name
HPS0381 : 1231A3  update : 2021/04/07
CommentHuman iPS cell line derived from healthy individual. HPS0379, HPS0380, HPS0381, HPS4293 was derived from the same person. Order Form (C-0042, C-0007 or C-0007p).
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach TERMS and CONDITIONS (non-for-profit) or TERMS and CONDITIONS (for-profit) to MTA (C-0007 or C-0007p) .
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamanaka, Shinya
Year of deposit 2013
Animal _human < Mammals
Genus Homo
Species sapiens
Race African/American
Gender Female
Age at sampling 29
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives StemFit AK02N
Antibiotics Free
Passage method TrypLE Select CTS : 0.5 mM EDTA/PBS(-) = 1 : 1
Coating dish 0.5 μg/cm^(2) Laminin-511 E8 (iMatrix511)
Culture information Passage cell No 2.6-26x10^(4) cells/60 mm dish
SC frequency Subculture : once/5-8 days, Medium Renewal : every day or every other day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells Free
Freeze medium StemFit AK02N + 10 μM Y-27632 + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XX,inv(9)(p12q13) 46,XX,inv(9)(p12q13) [20]
Others Use 10μM Y-27632 when passaging and thawing. Remove it next day.
deposit info
lot info
Reference information Reference 1
User's Publication 1

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4002  Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.  A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.  Sci Rep  2014  4:3594  PubMed ID: 24399248

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User's Publication
11055  Hayashi Y, Matsumoto J, Kumagai S, Morishita K, Xiang L, Kobori Y, Hori S, Suzuki M, Kanamori T, Hotta K, Sumaru K.  Automated Adherent Cell Elimination by a High-Speed Laser Mediated by a LightResponsive Polymer  Commun Biol  2018  1:218  PubMed ID: 30534610

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