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Cell No. : Cell Name
HPS0379 : 1201C1  update : 2024/07/09
CommentHuman iPS cell line derived from a healthy individual. HPS0379, HPS0380, HPS0381, HPS4293 was derived from the same person. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Year of deposit 2013
Animal _human < Mammals
Genus Homo
Species sapiens
Race African/American
Gender Female
Age at sampling 29
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_LJ37
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XX,inv(9)(p12q13) 46,XX,inv(9)(p12q13) [20]
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 1


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Reference
4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063   DOI: 10.1002/stem.1293

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User's Publication
21439  Terada E, Bamba Y, Takagaki M, Kawabata S, Tachi T, Nakamura H, Nishida T, Kishima H.  Silicate Microfiber Scaffolds Support the Formation and Expansion of the Cortical Neuronal Layer of Cerebral Organoids With a Sheet-Like Configuration.  Stem Cells Transl Med  2023    PubMed ID: 37843388   DOI: 10.1093/stcltm/szad066



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