Cell No. : Cell Name
HPS0360 : 648A1
update : 2025/01/16
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| Comment | Human iPS cell line derived from a healthy individual (peripheral blood)l. Established with episomal vector and no exogenous gene is present in the cell line. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment. Cryopreserved by vitrification method. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells. 2013;31:458-466) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Yamanaka, Shinya
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| Year of deposit |
2014
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Male
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| Age at sampling |
30s
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| Tissue |
peripheral blood
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_DQ30
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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Primate ES Cell Medium + 4ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-6 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
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(-)
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| Chromosome mode |
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46,XY[20] at passage No. 11
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
9
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| User's Publication |
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21827
Nakamura Y, Kobayashi H, Fukuda N, Tanaka S, Murata Y, Hatanaka Y, Haketa A, Tsunemi A, Chen L, Abe M.
Induced pluripotent stem cells derived renal tubular cells from a patient with pseudohypoparathyroidism and its response to parathyroid hormone stimulation.
Mol Biol Rep
2024
51(1):790
PubMed ID: 38990390
DOI: 10.1007/s11033-024-09751-8
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19717
Akaba Y, Takahashi S, Suzuki K, Kosaki K, Tsujimura K.
miR-514a promotes neuronal development in human iPSC-derived neurons
Front Cell Dev Biol
2023
11:1096463
PubMed ID: 36824367
DOI: 10.3389/fcell.2023.1096463
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17483
Nakatsu D, Kunishige R, Taguchi Y, Shinozaki-Narikawa N, Osaka K, Yokomizo K, Ishida M, Takei S, Yamasaki S, Hagiya K, Hattori K, Tsukamoto T, Murata M, Kano F.
BMP4-SMAD1/5/9-RUNX2 pathway activation inhibits neurogenesis and oligodendrogenesis in Alzheimer's patients' iPSCs in senescence-related conditions
Stem Cell Reports
2023
18(3):688-705
PubMed ID: 36764297
DOI: 10.1016/j.stemcr.2023.01.004
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20400
Suezawa T, Kanagaki S, Moriguchi K, Masui A, Nakao K, Toyomoto M, Tamai K, Mikawa R, Hirai T, Murakami K, Hagiwara M, Gotoh S.
Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids.
Stem Cell Reports
2021
PubMed ID: 34798066
DOI: 10.1016/j.stemcr.2021.10.015
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12074
Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.
Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.
Heliyon
2020
PubMed ID: 32154424
DOI: 10.1016/j.heliyon.2020.e03493
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12109
Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.
Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.
Fluids Barriers CNS
2020
PubMed ID: 32228708
DOI: 10.1186/s12987-020-00186-4
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13850
Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.
Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells
Fluids Barriers CNS
2020
17(1):36
PubMed ID: 32456699
DOI: 10.1186/s12987-020-00197-1
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4572
Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.
SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.
Nat Commun
2019
10(1):2175
PubMed ID: 31092818
DOI: 10.1038/s41467-019-09511-4
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13851
Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.
Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells
Heliyon
2019
6(1):e03067.
PubMed ID: 31909251
DOI: 10.1016/j.heliyon.2019.e03067
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