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Cell No. : Cell Name
HPS0360 : 648A1  update : 2023/06/07
CommentHuman iPS cell line derived from a healthy individual (peripheral blood)l. Established with episomal vector and no exogenous gene is present in the cell line. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment. Cryopreserved by vitrification method. Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells. 2013;31:458-466) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA.
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamanaka, Shinya
Year of deposit 2014
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 30s
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_DQ30
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY[20] at passage No. 11
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 1
User's Publication 9

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4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063   DOI: 10.1002/stem.1293

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User's Publication
21827  Nakamura Y, Kobayashi H, Fukuda N, Tanaka S, Murata Y, Hatanaka Y, Haketa A, Tsunemi A, Chen L, Abe M.  Induced pluripotent stem cells derived renal tubular cells from a patient with pseudohypoparathyroidism and its response to parathyroid hormone stimulation.  Mol Biol Rep  2024  51(1):790  PubMed ID: 38990390   DOI: 10.1007/s11033-024-09751-8
19717  Akaba Y, Takahashi S, Suzuki K, Kosaki K, Tsujimura K.  miR-514a promotes neuronal development in human iPSC-derived neurons  Front Cell Dev Biol  2023  11:1096463  PubMed ID: 36824367   DOI: 10.3389/fcell.2023.1096463
17483  Nakatsu D, Kunishige R, Taguchi Y, Shinozaki-Narikawa N, Osaka K, Yokomizo K, Ishida M, Takei S, Yamasaki S, Hagiya K, Hattori K, Tsukamoto T, Murata M, Kano F.  BMP4-SMAD1/5/9-RUNX2 pathway activation inhibits neurogenesis and oligodendrogenesis in Alzheimer's patients' iPSCs in senescence-related conditions  Stem Cell Reports  2023  18(3):688-705  PubMed ID: 36764297   DOI: 10.1016/j.stemcr.2023.01.004
20400  Suezawa T, Kanagaki S, Moriguchi K, Masui A, Nakao K, Toyomoto M, Tamai K, Mikawa R, Hirai T, Murakami K, Hagiwara M, Gotoh S.  Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids.  Stem Cell Reports  2021    PubMed ID: 34798066   DOI: 10.1016/j.stemcr.2021.10.015
12074  Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.  Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.  Heliyon  2020    PubMed ID: 32154424   DOI: 10.1016/j.heliyon.2020.e03493
12109  Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.  Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.  Fluids Barriers CNS  2020    PubMed ID: 32228708   DOI: 10.1186/s12987-020-00186-4
13850  Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.  Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells  Fluids Barriers CNS  2020  17(1):36  PubMed ID: 32456699   DOI: 10.1186/s12987-020-00197-1
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818   DOI: 10.1038/s41467-019-09511-4
13851  Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.  Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells  Heliyon  2019  6(1):e03067.  PubMed ID: 31909251   DOI: 10.1016/j.heliyon.2019.e03067

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