Cell No. : Cell Name
HPS0354 : 585A1
update : 2022/10/13
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| Comment | Human iPS cell line derived from a healthy individual (peripheral blood). Established with episomal vector. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells. 2013;31:458-466) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Yamanaka, Shinya
|
| Year of deposit |
2013
|
| Animal |
human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
30s
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| Tissue |
peripheral blood
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
|
| Vector |
pCXLE
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_DQ06
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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Primate ES Cell Medium + 4ng/ml human basic FGF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-6 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10^(6) cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Mycoplasma |
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(-)
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| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
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(-)
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| Chromosome mode |
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46,XY[20] at passage No. 16
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| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
1
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| Reference |
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20937
Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.
In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.
PLoS One
2022
17(11)6:e0275600
PubMed ID: 36378656
DOI: 10.1371/journal.pone.0275600
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4001
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.
An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.
Stem Cells
2013
31:458-66
PubMed ID: 23193063
DOI: 10.1002/stem.1293
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