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Cell No. : Cell Name
HPS0354 : 585A1  update : 2022/04/05
CommentHuman iPS cell line derived from a healthy individual (peripheral blood). Established with episomal vector. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells. 2013;31:458-466) designated by the DEPOSITOR is required. 3) Attach TERMS and CONDITIONS to MTA.
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Year of deposit 2013
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 30s
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
Vector pCXLE
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_DQ06
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY[20] at passage No. 16
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 1


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Reference
4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063

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User's Publication
20400  Suezawa T, Kanagaki S, Moriguchi K, Masui A, Nakao K, Toyomoto M, Tamai K, Mikawa R, Hirai T, Murakami K, Hagiwara M, Gotoh S.  Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids.  Stem Cell Reports  2021    PubMed ID: 34798066



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