Cell No. : Cell Name
HPS0354 : 585A1
update : 2025/01/16
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| Comment | Human iPS cell line derived from a healthy individual (peripheral blood). Established with episomal vector. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells. 2013;31:458-466) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Yamanaka, Shinya
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| Year of deposit |
2013
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Male
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| Age at sampling |
30s
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| Tissue |
peripheral blood
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
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| Vector |
pCXLE
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_DQ06
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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Primate ES Cell Medium + 4ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-6 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
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(-)
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| Chromosome mode |
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46,XY[20] at passage No. 16
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
8
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| Reference |
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20937
Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.
In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.
PLoS One
2022
17(11)6:e0275600
PubMed ID: 36378656
DOI: 10.1371/journal.pone.0275600
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4001
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.
An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.
Stem Cells
2013
31:458-66
PubMed ID: 23193063
DOI: 10.1002/stem.1293
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| User's Publication |
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21938
Okano M, Yasuda M, Shimomura Y, Matsuoka Y, Shirouzu Y, Fujioka T, Kyo M, Tsuji S, Kaneko K, Hitomi H.
Citrin-deficient patient-derived induced pluripotent stem cells as a pathological liver model for congenital urea cycle disorders.
Mol Genet Metab Rep
2024
40:101096
PubMed ID: 38872960
DOI: 10.1016/j.ymgmr.2024.101096
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22440
Uno N, Miyamoto H, Yamazaki K, Egawa M, Kobayashi H, Kazuki K, Osaki M, Suzuki T, Hamamichi S, Oshimura M, Tomizuka K, Kazuki Y.
Microcell-mediated chromosome transfer between non-identical human iPSCs.
Mol Ther Nucleic Acids
2024
35(4):102382
PubMed ID: 39634136
DOI: 10.1016/j.omtn.2024.102382
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19717
Akaba Y, Takahashi S, Suzuki K, Kosaki K, Tsujimura K.
miR-514a promotes neuronal development in human iPSC-derived neurons
Front Cell Dev Biol
2023
11:1096463
PubMed ID: 36824367
DOI: 10.3389/fcell.2023.1096463
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21283
Abdalkader R, Chaleckis R, Fujita T.
Early Differentiation Signatures in Human Induced Pluripotent Stem Cells Determined by Non-Targeted Metabolomics Analysis.
Metabolites
2023
13(6)
PubMed ID: 37367864
DOI: 10.3390/metabo13060706
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21311
Kaneko H, Kaitsuka T, Tomizawa K.
Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.
Cell Mol Life Sci
2023
80(8):200
PubMed ID: 37421441
DOI: 10.1007/s00018-023-04847-z
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21405
Murata H, Phoo MTZ, Ochi T, Tomonobu N, Yamamoto KI, Kinoshita R, Miyazaki I, Nishibori M, Asanuma M, Sakaguchi M.
Phosphorylated SARM1 is involved in the pathological process of rotenone-induced neurodegeneration.
J Biochem
2023
PubMed ID: 37725528
DOI: 10.1093/jb/mvad068
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21492
Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.
Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
Cell Rep
2023
113431
PubMed ID: 38039961
DOI: 10.1016/j.celrep.2023.113431
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20400
Suezawa T, Kanagaki S, Moriguchi K, Masui A, Nakao K, Toyomoto M, Tamai K, Mikawa R, Hirai T, Murakami K, Hagiwara M, Gotoh S.
Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids.
Stem Cell Reports
2021
PubMed ID: 34798066
DOI: 10.1016/j.stemcr.2021.10.015
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