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Cell No. : Cell Name
HPS0354 : 585A1  update : 2023/11/21
CommentHuman iPS cell line derived from a healthy individual (peripheral blood). Established with episomal vector. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells. 2013;31:458-466) designated by the DEPOSITOR is required. 3) Attach Appendix to MTA.
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamanaka, Shinya
Year of deposit 2013
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 30s
Tissue peripheral blood
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
Vector pCXLE
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_DQ06
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46,XY[20] at passage No. 16
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 2
User's Publication 6

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20937  Tsujimoto H, Katagiri N, Ijiri Y, Sasaki B, Kobayashi Y, Mima A, Ryosaka M, Furuyama K, Kawaguchi Y, Osafune K.  In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor cells.  PLoS One  2022  17(11)6:e0275600  PubMed ID: 36378656   DOI: 10.1371/journal.pone.0275600
4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063   DOI: 10.1002/stem.1293

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User's Publication
19717  Akaba Y, Takahashi S, Suzuki K, Kosaki K, Tsujimura K.  miR-514a promotes neuronal development in human iPSC-derived neurons  Front Cell Dev Biol  2023  11:1096463  PubMed ID: 36824367   DOI: 10.3389/fcell.2023.1096463
21283  Abdalkader R, Chaleckis R, Fujita T.  Early Differentiation Signatures in Human Induced Pluripotent Stem Cells Determined by Non-Targeted Metabolomics Analysis.  Metabolites  2023  13(6)  PubMed ID: 37367864   DOI: 10.3390/metabo13060706
21311  Kaneko H, Kaitsuka T, Tomizawa K.  Artificial induction of circadian rhythm by combining exogenous BMAL1 expression and polycomb repressive complex 2 inhibition in human induced pluripotent stem cells.  Cell Mol Life Sci  2023  80(8):200  PubMed ID: 37421441   DOI: 10.1007/s00018-023-04847-z
21405  Murata H, Phoo MTZ, Ochi T, Tomonobu N, Yamamoto KI, Kinoshita R, Miyazaki I, Nishibori M, Asanuma M, Sakaguchi M.  Phosphorylated SARM1 is involved in the pathological process of rotenone-induced neurodegeneration.  J Biochem  2023    PubMed ID: 37725528   DOI: 10.1093/jb/mvad068
21492  Mae SI, Hattanda F, Morita H, Nozaki A, Katagiri N, Ogawa H, Teranaka K, Nishimura Y, Kudoh A, Yamanaka S, Matsuse K, Ryosaka M, Watanabe A, Soga T, Nishio S, Osafune K.  Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.  Cell Rep  2023  113431  PubMed ID: 38039961   DOI: 10.1016/j.celrep.2023.113431
20400  Suezawa T, Kanagaki S, Moriguchi K, Masui A, Nakao K, Toyomoto M, Tamai K, Mikawa R, Hirai T, Murakami K, Hagiwara M, Gotoh S.  Disease modeling of pulmonary fibrosis using human pluripotent stem cell-derived alveolar organoids.  Stem Cell Reports  2021    PubMed ID: 34798066   DOI: 10.1016/j.stemcr.2021.10.015

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