Cell No. : Cell Name
HPS0352 : VCP-#2
update : 2024/04/05
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| Comment | Disease specific iPS cell line derived from a patient : Inclusion body myositis. HPS0351 and HPS0353 were derived from the same patient. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please access to the following URL and consult 'iPS Academia Japan, Inc.' (https://ips-cell.net/e/index.php) prior to the utilization of iPS cells. 2) Collaboration with the depositor is necessary. 3) The DEPOSITOR developed the BIOLOGICAL RESOURCE (iPS cell lines) with the cells obtained from the NIGMS Human Genetic Cell Repository (the Repository ID numbers is GM22394) . The RECIPIENT who obtained the BIOLOGICAL RESOURCE from the RIKEN BRC must acknowledge the NIGMS Human Genetic Cell Repository and the cell line number in any publications or presentations based on the utilization of the NIGMS Repository Sample as follows:'HPS0351 was derived from cell line GM22394 from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.' 4) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Caucasian
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| Gender |
Male
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| Age at sampling |
30s
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| Tissue |
skin
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| Disease name |
Inclusion body myopathy
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
pDON-5 Oct3/4-Sox2, pDON-5 Klf4, pDON-5 LIN28-NANOG
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_T898
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.08mM NEAA + 0.1mM 2-Mercaptoethanol + 4ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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