Back Back
Cell No. : Cell Name
HPS0351 : VCP-#1  update : 2024/01/05
CommentDisease specific iPS cell line derived from a patient : Inclusion body myositis. HPS0352 and HPS0353 were derived from the same patient. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please access to the following URL and consult 'iPS Academia Japan, Inc.' ( prior to the utilization of iPS cells. 2) Collaboration with the depositor is necessary. 3) The DEPOSITOR developed the BIOLOGICAL RESOURCE (iPS cell lines) with the cells obtained from the NIGMS Human Genetic Cell Repository (the Repository ID numbers is GM22394) . The RECIPIENT who obtained the BIOLOGICAL RESOURCE from the RIKEN BRC must acknowledge the NIGMS Human Genetic Cell Repository and the cell line number in any publications or presentations based on the utilization of the NIGMS Repository Sample as follows:'HPS0351 was derived from cell line GM22394 from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research.' 4) Contact Us : RIKEN Cell Bank
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Animal _human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 30s
Tissue skin
Disease name Inclusion body myopathy
Classification iPS
Recombinant recombinant
Exogene pDON-5 Oct3/4-Sox2, pDON-5 Klf4, pDON-5 LIN28-NANOG
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T897
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.08mM NEAA + 0.1mM 2-Mercaptoethanol + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 0
User's Publication 0

To topTop

To topTop
User's Publication

Back Back Return Top Page