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Cell No. : Cell Name
HPS0337 :  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : X-linked Chronic granulomatous disease (X-CGD), gp91phox-deficiency). HPS0338 and HPS0339 were derived from the same patient. iPS cells were created using the Sendai virus vector. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) In-depth genome sequencing analysis is not applicable. 4) Contact Us : RIKEN Cell Bank
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Another name XCGD-iPSC
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 1-9 years
Tissue peripheral blood, CD34 positive
Disease name X-linked Chronic granulomatous disease (X-CGD)
Classification iPS
Recombinant recombinant
Exogene Sendai Virus Vector (Oct3/4, Sox2, Klf4, c-Myc)
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_1T00
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 20% KSR + 1mM CaCl2+PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
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lot info
Relational File deposit infolot info
Reference information Reference 2
User's Publication 0

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17178  Lin HT, Okumura T, Yatsuda Y, Ito S, Nakauchi H, Otsu M.  Application of Droplet Digital PCR for Estimating Vector Copy Number States in Stem Cell Gene Therapy  Hum Gene Ther Methods  2016  27(5):197-208  PubMed ID: 27763786   DOI: 10.1089/hgtb.2016.059
17177  Lin HT, Masaki H, Yamaguchi T, Wada T, Yachie A, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H, Otsu M.  An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils  Mol Ther Methods Clin Dev  2015  2:15046  PubMed ID: 26682238   DOI: 10.1038/mtm.2015.46

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