Cell No. : Cell Name
HPS0336 :
update : 2023/06/07
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| Comment | Disease specific iPS cell line derived from a patient : Chronic granulomatous disease (CGD), p47phox-deficiency. HPS0334 and HPS0335 were derived from the same patient. iPS cells were created using the Sendai virus vector. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) In-depth genome sequencing analysis is not applicable. 4) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Female
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| Age at sampling |
30s
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| Tissue |
peripheral blood, CD34 positive
|
| Disease name |
Chronic granulomatous disease (CGD)
|
| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Sendai Virus Vector (Oct3/4, Sox2, Klf4, c-Myc）
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|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_1R67
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
|
DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin + 20% KSR + 1mM CaCl2+PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
|
X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.0-1.5 x 10 6 cells/100 mm dish
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| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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