Cell No. : Cell Name
HPS0332 :
update : 2023/08/23
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| Comment | Disease specific iPS cell line derived from a patient : Hypophosphatasia, perinatal type. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) For profit organization is limited to academic use. |
| Remarks | Use of the material requires approval by institutional review board for research ethics. |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Age at sampling |
1-12 months
|
| Tissue |
bone marrow
|
| Disease name |
Hypophosphatasia, perinatal type
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
pMXs-Oct3/4, pMXs-Sox2, pMXs-Klf4, pMXs-c-Myc
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_1R58
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
|
DMEM/HamF12 (2.5mM GlutaMAX) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
|
| Antibiotics |
|
Free
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|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
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| Feeder treatment |
|
X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
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| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
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| Mycoplasma/Acholeplasma |
|
(-)
|
| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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