細胞番号 : 細胞名
HPS0331 : 610B1
update : 2023/06/07
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| 細胞特性(Comment:英) | Human iPS cell line derived from a healthy individual (cord blood) . HPS0330, HPS0331 was derived from the same person. Cryopreserved by vitrification method. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| 細胞特性(日) | ヒト人工多能性幹（iPS）細胞株。HPS0330, HPS0331は同一人由来。ガラス化法（Vitrification法）保存。営利機関は、公益財団法人京都大学iPS細胞研究財団にお問い合わせください。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA. |
| 使用条件(日) | 1) 利用者は学術機関に所属している者に限る。2) 別紙1を提供同意書に添付すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0041.pdf
同意書(非営利学術目的)C-0003.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Yamanaka, Shinya
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| 寄託日 |
2014
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Male
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| 採取組織 |
cord blood
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| 細胞分類 |
iPS
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
Oct3/4, Sox2, Klf4, L-Myc, Lin28
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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| Cellosaurus(Expasy) |
CVCL_DQ14
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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Primate ES Cell Medium + 4ng/ml human basic FGF
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| 培養容器のコーティング |
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0.1% gelatin coated dish
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継代密度 |
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1 : 3-5 split
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| 継代・培地交換頻度 |
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Subculture : once/4-6 days, Medium Renewal : every day
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| フィーダー細胞 |
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SNL 76/7
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| フィーダー細胞の処理方法 |
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X-rays 5000R (or MMC)
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| フィーダー細胞の播種数 |
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1.5-2.5 x 10 6 cells/100 mm dish
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| 凍結培地 |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| 凍結方法 |
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Vitrification
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| マイコプラズマ/アコレプラズマ |
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(-)
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| ウィルス（HIV) |
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Undetected
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| ウィルス（HTLV-1) |
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(-)
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| 染色体検査 |
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46XY(20)
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| その他の細胞情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
1件
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| Reference(日) |
0件
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| 利用者成果(英) |
11件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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19980
Nakanishi T, Yamazaki M, Tachikawa K, Ueta A, Kawai M, Ozono K, Michigami T.
Complex intrinsic abnormalities in osteoblast lineage cells of X-linked hypophosphatemia: Analysis of human iPS cell models generated by CRISPR/Cas9-mediated gene ablation
Bone
2024
Feb 6:117044
PubMed ID: 38331306
DOI: 10.1016/j.bone.2024.117044
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21613
Tanaka Y, Mizuguchi R, Koseki N, Suzuki H, Suzuki T.
Quality assessment of enzymatic methyl-seq library constructed using crude cell lysate.
Biochem Biophys Res Commun
2024
696:149488
PubMed ID: 38219485
DOI: 10.1016/j.bbrc.2024.149488
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21055
Toya A, Fukada M, Aoki E, Matsuki T, Ueda M, Eda S, Hashizume Y, Iio A, Masaki S, Nakayama A.
The distribution of neuroligin4, an autism-related postsynaptic molecule, in the human brain.
Mol Brain
2023
16(1):20
PubMed ID: 36747195
DOI: 10.1186/s13041-023-00999-y
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21395
Tanaka Y, Kadota S, Zhao J, Kobayashi H, Okano S, Izumi M, Honda Y, Ichimura H, Shiba N, Uemura T, Wada Y, Chuma S, Nakada T, Tohyama S, Fukuda K, Yamada M, Seto T, Kuwahara K, Shiba Y.
Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin.
Stem Cell Res Ther
2023
14(1):240
PubMed ID: 37679796
DOI: 10.1186/s13287-023-03468-4
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21520
Shiba N, Yang X, Sato M, Kadota S, Suzuki Y, Agata M, Nagamine K, Izumi M, Honda Y, Koganehira T, Kobayashi H, Ichimura H, Chuma S, Nakai J, Tohyama S, Fukuda K, Miyazaki D, Nakamura A, Shiba Y.
Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.
Mol Ther Nucleic Acids
2023
34:102060
PubMed ID: 38028197
DOI: 10.1016/j.omtn.2023.102060
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12074
Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.
Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.
Heliyon
2020
PubMed ID: 32154424
DOI: 10.1016/j.heliyon.2020.e03493
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12109
Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.
Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.
Fluids Barriers CNS
2020
PubMed ID: 32228708
DOI: 10.1186/s12987-020-00186-4
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13850
Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.
Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells
Fluids Barriers CNS
2020
17(1):36
PubMed ID: 32456699
DOI: 10.1186/s12987-020-00197-1
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11589
Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.
Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.
Biochem. Biophys. Res. Commun.
2019
515(4):672-678
PubMed ID: 31178142
DOI: 10.1016/j.bbrc.2019.05.179
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13851
Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.
Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells
Heliyon
2019
6(1):e03067.
PubMed ID: 31909251
DOI: 10.1016/j.heliyon.2019.e03067
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7015
Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.
Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.
Stem Cell Reports
2017
9:1441-1449
PubMed ID: 29107592
DOI: 10.1016/j.stemcr.2017.09.021
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