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Cell No. : Cell Name
HPS0331 : 610B1  update : 2021/04/07
CommentHuman iPS cell line derived from a healthy individual (cord blood) . HPS0330, HPS0331 was derived from the same person. Order Form (C-0042, C-0034). Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0034.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Year of deposit 2014
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue cord blood
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46XY(20)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 6


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Reference
4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063

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User's Publication
12074  Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.  Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.  Heliyon  2020    PubMed ID: 32154424
12109  Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.  Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.  Fluids Barriers CNS  2020    PubMed ID: 32228708
13850  Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.  Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells  Fluids Barriers CNS  2020  17(1):36  PubMed ID: 32456699
11589  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Biochem. Biophys. Res. Commun.  2019  515(4):672-678  PubMed ID: 31178142
13851  Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.  Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells  Heliyon  2019  6(1):e03067.  PubMed ID: 31909251
7015  Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.  Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.  Stem Cell Reports  2017  9:1441-1449  PubMed ID: 29107592



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