Cell No. : Cell Name
HPS0331 : 610B1
update : 2020/12/17
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Comment | Human iPS cell line derived from a healthy individual (cord blood) . HPS0330, HPS0331 was derived from the same person. Order Form (C-0042, C-0034). Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
Comment from the depositor | |
Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) . |
Remarks | |
Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Yamanaka, Shinya
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Year of deposit |
2014
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Animal |
human
< Mammals
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Genus |
Homo
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Species |
sapiens
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Gender |
Male
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Tissue |
cord blood
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Classification |
iPS
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Recombinant |
recombinant
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Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28
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|
Lifespan |
infinite
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Morphology |
ES-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Medium and additives |
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Primate ES Cell Medium + 4ng/ml human basic FGF
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Antibiotics |
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Free
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Passage method |
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0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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Temperature |
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37
℃
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CO2 concentration |
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5
%
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Feeder cells |
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SNL 76/7
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Feeder treatment |
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X-rays 5000R (or MMC)
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Feeder seed cells |
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1.5-2.5 x 10^(6) cells/100 mm dish
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Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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Freezing method |
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Vitrification
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Mycoplasma |
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(-)
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STR(human) |
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OK
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Virus (HIV) |
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Undetected
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Virus (HTLV-1) |
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(-)
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Chromosome mode |
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46XY(20)
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Images |
deposit info | lot info |
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Relational File |
deposit info | lot info |
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Reference information |
Reference |
1
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User's Publication |
4
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User's Publication |
12074
Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.
Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.
Heliyon
2020
PubMed ID: 32154424
|
12109
Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.
Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.
Fluids Barriers CNS
2020
PubMed ID: 32228708
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11589
Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.
Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.
Biochem. Biophys. Res. Commun.
2019
515(4):672-678
PubMed ID: 31178142
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7015
Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.
Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.
Stem Cell Reports
2017
9:1441-1449
PubMed ID: 29107592
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