Cell No. : Cell Name
HPS0331 : 610B1
update : 2022/02/03
|
| Comment | Human iPS cell line derived from a healthy individual (cord blood) . HPS0330, HPS0331 was derived from the same person. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Yamanaka, Shinya
|
| Year of deposit |
2014
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Tissue |
cord blood
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_DQ14
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
Primate ES Cell Medium + 4ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage ratio |
|
1 : 3-5 split
|
| SC frequency |
|
Subculture : once/4-6 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.5-2.5 x 10^(6) cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Chromosome mode |
|
46XY(20)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Relational File |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
6
|
| User's Publication |
|
12074
Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.
Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.
Heliyon
2020
PubMed ID: 32154424
|
|
12109
Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.
Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.
Fluids Barriers CNS
2020
PubMed ID: 32228708
|
|
13850
Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.
Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells
Fluids Barriers CNS
2020
17(1):36
PubMed ID: 32456699
|
|
11589
Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.
Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.
Biochem. Biophys. Res. Commun.
2019
515(4):672-678
PubMed ID: 31178142
|
|
13851
Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.
Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells
Heliyon
2019
6(1):e03067.
PubMed ID: 31909251
|
|
7015
Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.
Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.
Stem Cell Reports
2017
9:1441-1449
PubMed ID: 29107592
|
Back
Top
CELL BANK Website
SDS_DAP(MEM)_Japanese.pdf