Cell No. : Cell Name
HPS0331 : 610B1
update : 2020/12/17
|
| Comment | Human iPS cell line derived from a healthy individual (cord blood) . HPS0330, HPS0331 was derived from the same person. Order Form (C-0042, C-0034). Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) . |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
|
|
|
Basic information
|
Depositor |
Yamanaka, Shinya
|
| Year of deposit |
2014
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Tissue |
cord blood
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
Primate ES Cell Medium + 4ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage ratio |
|
1 : 3-5 split
|
| SC frequency |
|
Subculture : once/4-6 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.5-2.5 x 10^(6) cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Chromosome mode |
|
46XY(20)
|
| Images |
deposit info | lot info |
|
|
|
| Relational File |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
4
|
| User's Publication |
|
12074
Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.
Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.
Heliyon
2020
PubMed ID: 32154424
|
|
12109
Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.
Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.
Fluids Barriers CNS
2020
PubMed ID: 32228708
|
|
11589
Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.
Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.
Biochem. Biophys. Res. Commun.
2019
515(4):672-678
PubMed ID: 31178142
|
|
7015
Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.
Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.
Stem Cell Reports
2017
9:1441-1449
PubMed ID: 29107592
|
Back
Top
CELL BANK Website
SDS_DAP(MEM)_Japanese.pdf