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Cell No. : Cell Name
HPS0331 : 610B1  update : 2023/06/07
CommentHuman iPS cell line derived from a healthy individual (cord blood) . HPS0330, HPS0331 was derived from the same person. Cryopreserved by vitrification method. Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA.
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Yamanaka, Shinya
Year of deposit 2014
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue cord blood
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_DQ14
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46XY(20)
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 1
User's Publication 11

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4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063   DOI: 10.1002/stem.1293

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User's Publication
19980  Nakanishi T, Yamazaki M, Tachikawa K, Ueta A, Kawai M, Ozono K, Michigami T.  Complex intrinsic abnormalities in osteoblast lineage cells of X-linked hypophosphatemia: Analysis of human iPS cell models generated by CRISPR/Cas9-mediated gene ablation  Bone  2024  Feb 6:117044  PubMed ID: 38331306   DOI: 10.1016/j.bone.2024.117044
21613  Tanaka Y, Mizuguchi R, Koseki N, Suzuki H, Suzuki T.  Quality assessment of enzymatic methyl-seq library constructed using crude cell lysate.  Biochem Biophys Res Commun  2024  696:149488  PubMed ID: 38219485   DOI: 10.1016/j.bbrc.2024.149488
21055  Toya A, Fukada M, Aoki E, Matsuki T, Ueda M, Eda S, Hashizume Y, Iio A, Masaki S, Nakayama A.  The distribution of neuroligin4, an autism-related postsynaptic molecule, in the human brain.  Mol Brain  2023  16(1):20  PubMed ID: 36747195   DOI: 10.1186/s13041-023-00999-y
21395  Tanaka Y, Kadota S, Zhao J, Kobayashi H, Okano S, Izumi M, Honda Y, Ichimura H, Shiba N, Uemura T, Wada Y, Chuma S, Nakada T, Tohyama S, Fukuda K, Yamada M, Seto T, Kuwahara K, Shiba Y.  Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin.  Stem Cell Res Ther  2023  14(1):240  PubMed ID: 37679796   DOI: 10.1186/s13287-023-03468-4
21520  Shiba N, Yang X, Sato M, Kadota S, Suzuki Y, Agata M, Nagamine K, Izumi M, Honda Y, Koganehira T, Kobayashi H, Ichimura H, Chuma S, Nakai J, Tohyama S, Fukuda K, Miyazaki D, Nakamura A, Shiba Y.  Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.  Mol Ther Nucleic Acids  2023  34:102060  PubMed ID: 38028197   DOI: 10.1016/j.omtn.2023.102060
12074  Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.  Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.  Heliyon  2020    PubMed ID: 32154424   DOI: 10.1016/j.heliyon.2020.e03493
12109  Aoki H, Yamashita M, Hashita T, Iwao T, Matsunaga T.  Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity.  Fluids Barriers CNS  2020    PubMed ID: 32228708   DOI: 10.1186/s12987-020-00186-4
13850  Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.  Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells  Fluids Barriers CNS  2020  17(1):36  PubMed ID: 32456699   DOI: 10.1186/s12987-020-00197-1
11589  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Biochem. Biophys. Res. Commun.  2019  515(4):672-678  PubMed ID: 31178142   DOI: 10.1016/j.bbrc.2019.05.179
13851  Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.  Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells  Heliyon  2019  6(1):e03067.  PubMed ID: 31909251   DOI: 10.1016/j.heliyon.2019.e03067
7015  Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.  Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.  Stem Cell Reports  2017  9:1441-1449  PubMed ID: 29107592   DOI: 10.1016/j.stemcr.2017.09.021

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