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Cell No. : Cell Name
HPS0328 : 606A1  update : 2021/04/06
CommentHuman iPS cell line derived from a healthy individual (cord blood) . HPS0328, HPS0329 was derived from the same person. Order Form (C-0042, C-0034). Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0034.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamanaka, Shinya
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cord blood
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Chromosome mode 46XX(20)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 5


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Reference
4001  Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S.  An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.  Stem Cells  2013  31:458-66  PubMed ID: 23193063

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User's Publication
12074  Aoki H, Yamashita M, Hashita T, Ogami K, Hoshino S, Iwao T, Matsunaga T.  Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β.  Heliyon  2020    PubMed ID: 32154424
13370  Xu L, Hanamatsu H, Homan K, Onodera T, Miyazaki T, Furukawa JI, Hontani K, Tian Y, Baba R, Iwasaki N.  Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes.  Biomolecules  2020    PubMed ID: 33271874
13850  Yamashita M, Aoki H, Hashita T, Iwao T, Matsunaga T.  Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells  Fluids Barriers CNS  2020  17(1):36  PubMed ID: 32456699
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818
13851  Grassi DA, Brattås PL, Jönsson ME, Atacho D, Karlsson O, Nolbrant S, Parmar M, Jakobsson J.  Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells  Heliyon  2019  6(1):e03067.  PubMed ID: 31909251



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