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Cell No. : Cell Name
HPS0266 :  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : Adenosine deaminase deficiency (ADA). HPS0265 and HPS0267 were derived from the same patient. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) In-depth genome sequencing analysis is not applicable. 3) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 10s
Tissue bone marrow, stromal
Disease name adenosine deaminase deficiency (ADA)
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc
Vector pMXs-Oct3/4, pMXs-Sox2, pMXs-Klf4, pMXs-c-Myc
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_6C85
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 20% KSR + 1mM CaCl2+PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10 5 cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 0


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