Cell No. : Cell Name
HPS0265 :
update : 2023/06/07
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| Comment | Disease specific iPS cell line derived from a patient : Adenosine deaminase deficiency (ADA). HPS0266 and HPS0267 were derived from the same patient. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) In-depth genome sequencing analysis is not applicable. 3) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Male
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| Age at sampling |
10s
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| Tissue |
bone marrow, stromal
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| Disease name |
adenosine deaminase deficiency (ADA)
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, c-Myc
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| Vector |
pMXs-Oct3/4, pMXs-Sox2, pMXs-Klf4, pMXs-c-Myc
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_T940
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 20% KSR + 1mM CaCl2+PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10 5 cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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