Cell No. : Cell Name
HPS0251 :
update : 2025/01/16
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| Comment | Disease specific iPS cell line derived from a patient : Amyotrophic lateral sclerosis (ALS). HPS0251, HPS1719 were derived from the same patient. Analysis of causal genetic changes. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies) . 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 4) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
Approval Form(C-0057.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Year of deposit |
2012
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
40s
|
| Tissue |
skin
|
| Disease name |
amyotrophic lateral sclerosis (ALS)
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_VM60
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
Primate ES Cell Medium + 4ng/ml human basic FGF
|
| Antibiotics |
|
Free
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|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
|
0.1% gelatin coated dish
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Culture information
|
Passage ratio |
|
1 : 3-5 split
|
| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
SNL 76/7
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| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
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1.5-2.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
|
| User's Publication |
1
|
| User's Publication |
|
11123
Tsuburaya N, Homma K, Higuchi T, Balia A, Yamakoshi H, Shibata N, Nakamura S, Nakagawa H, Ikeda SI, Umezawa N, Kato N, Yokoshima S, Shibuya M, Shimonishi M, Kojima H, Okabe T, Nagano T, Naguro I, Imamura K, Inoue H, Fujisawa T, Ichijo H.
A small-molecule inhibitor of SOD1-Derlin-1 interaction ameliorates pathology in an ALS mouse model.
Nat Commun
2018
9(1):2668
PubMed ID: 29991716
DOI: 10.1038/s41467-018-05127-2
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