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Cell No. : Cell Name
HPS0223 : Nips-B2  update : 2021/04/05
CommentHuman iPS cell line. iPS cells were created using the Sendai virus vector. Past history : atopic asthma. Order Form(C-0042, C-0007 or C-0007p).
Comment from the depositor
Terms and conditions1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS One. 2012;7:e42855.) designated by the DEPOSITOR is required.
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Noguchi, Emiko
Originator Hamada, Y., Ono, M.
Year of deposit 2012
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 43 years
Tissue Nasal Epithelial cells
Disease name atopic asthma
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Chromosome mode 46XX(20)
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 1
User's Publication 7

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5456  Ono M, Hamada Y, Horiuchi Y, Matsuo-Takasaki M, Imoto Y, Satomi K, Arinami T, Hasegawa M, Fujioka T, Nakamura Y, Noguchi E.  Generation of induced pluripotent stem cells from human nasal epithelial cells using a Sendai virus vector.  PLoS One.  2012  7(8):e42855  PubMed ID: 22912751

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User's Publication
13411  Yasui R, Sekine K, Yamaguchi K, Furukawa Y, Taniguchi H.  Robust parameter design of human induced pluripotent stem cell differentiation protocols defines lineage-specific induction of anterior-posterior gut tube endodermal cells.  Stem Cells  2021    PubMed ID: 33400835
12000  Zujur D, Kanke K, Onodera S, Tani S, Lai J, Azuma T, Xin X, Lichtler AC, Rowe DW, Saito T, Tanaka S, Masaki H, Nakauchi H, Chung UI, Hojo H, Ohba S.  Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.  Regen Ther  2020    PubMed ID: 31988991
13175  Morita N, Onodera S, Nakamura Y, Nakamura T, Takahashi SI, Nomura T, Azuma T.  Keratinocytes from Gorlin Syndrome-induced pluripotent stem cells are resistant against UV radiation.  Med Mol Morphol  2020    PubMed ID: 32816116
13624  Shuhei Soeda, Ryo Saito, Ai Fujii, Shusei Tojo, Yuka Tokumura and Hideo Taniura  Abnormal DNA Methylation in Pluripotent Stem Cells from a Patient with Prader-Willi Syndrome Results in Neuronal Differentiation Defects  STEM Cell Research  2020   
10593  Hasegawa D, Ochiai-Shino H, Onodera S, Nakamura T, Saito A, Onda T, Watanabe K, Nishimura K, Ohtaka M, Nakanishi M, Kosaki K, Yamaguchi A, Shibahara T, Azuma T.  Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.  PLoS ONE  2017  12:e0186879  PubMed ID: 29088246
14039  Sato Y, Kobayashi H, Higuchi T, Shimada Y, Ida H, Ohashi T.  Metabolomic Profiling of Pompe Disease-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Reveals That Oxidative Stress Is Associated with Cardiac and Skeletal Muscle Pathology  Stem Cells Transl Med  2017  6(1):31-39  PubMed ID: 28170191
7266  Sato Y, Kobayashi H, Higuchi T, Shimada Y, Era T, Kimura S, Eto Y, Ida H, Ohashi T.  Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient.  Mol Ther Methods Clin Dev  2015  2:15023  PubMed ID: 26199952

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