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Cell No. : Cell Name
HPS0223 : Nips-B2  update : 2019/09/06
CommentHuman iPS cell line. iPS cells were created using the Sendai virus vector. Past history : atopic asthma. Order Form(C-0042, C-0007 or C-0007p).
Comment from the depositor
Terms and conditions1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (PLoS One. 2012;7:e42855.) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Noguchi, Emiko
Originator Hamada, Y., Ono, M.
Year of deposit 2012
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 43 years
Tissue Nasal Epithelial cells
Disease name atopic asthma
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Chromosome mode 46XX(20)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 3


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Reference
5456  Ono M, Hamada Y, Horiuchi Y, Matsuo-Takasaki M, Imoto Y, Satomi K, Arinami T, Hasegawa M, Fujioka T, Nakamura Y, Noguchi E.  Generation of induced pluripotent stem cells from human nasal epithelial cells using a Sendai virus vector.  PLoS One.  2012  7(8):e42855  PubMed ID: 22912751

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User's Publication
12000  Zujur D, Kanke K, Onodera S, Tani S, Lai J, Azuma T, Xin X, Lichtler AC, Rowe DW, Saito T, Tanaka S, Masaki H, Nakauchi H, Chung UI, Hojo H, Ohba S.  Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.  Regen Ther  2020    PubMed ID: 31988991
10593  Hasegawa D, Ochiai-Shino H, Onodera S, Nakamura T, Saito A, Onda T, Watanabe K, Nishimura K, Ohtaka M, Nakanishi M, Kosaki K, Yamaguchi A, Shibahara T, Azuma T.  Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.  PLoS ONE  2017  12:e0186879  PubMed ID: 29088246
7266  Sato Y, Kobayashi H, Higuchi T, Shimada Y, Era T, Kimura S, Eto Y, Ida H, Ohashi T.  Disease modeling and lentiviral gene transfer in patient-specific induced pluripotent stem cells from late-onset Pompe disease patient.  Mol Ther Methods Clin Dev  2015  2:15023  PubMed ID: 26199952



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