Cell No. : Cell Name
HPS0177 :
update : 2020/07/03
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Comment | Disease specific iPS cell line derived from a patient : Pompe’s disease. HPS0175 and HPS0176 were derived from the same patient. iPS cells were created using the Sendai virus vector. Order Form (C-0042, C-0057, C-0007 or C-0007p). |
Comment from the depositor | |
Terms and conditions | 1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the APPROVER. 4) Contact Us : RIKEN Cell Bank |
Remarks | |
Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Animal |
human
< Mammals
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Genus |
Homo
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Species |
sapiens
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Race |
Japanese
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Gender |
Female
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Age at sampling |
1-9 years
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Tissue |
skin
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Disease name |
Glycogen storage disease
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Classification |
iPS
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Recombinant |
recombinant
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Exogene |
SeV18+HS-OCT3/4/TSΔF, SeV18+HS-SOX2/TSΔF, SeV18+HS-KLF4/TSΔF, SeV18(HNL)c-MYCQC/TSΔF
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Lifespan |
infinite
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Morphology |
ES-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Medium and additives |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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Antibiotics |
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Free
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Passage method |
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0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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Temperature |
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37
℃
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CO2 concentration |
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3
%
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Feeder cells |
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MEF
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Feeder treatment |
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X-rays 5000R (or MMC)
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Feeder seed cells |
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3-5 x 10^(5) cells/60 mm dish
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Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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Freezing method |
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Vitrification
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Mycoplasma |
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(-)
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STR(human) |
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OK
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Images |
deposit info | lot info |
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Relational File |
deposit info | lot info |
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Reference information |
Reference |
0
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User's Publication |
1
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