Cell No. : Cell Name
HPS0164 :
update : 2019/09/06
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| Comment | Disease specific iPS cell line derived from a patient : Muscular dystrophy, Duchenne type. iPS cells were created using the Sendai virus vector. Order Form (C-0042, C-0057, C-0007 or C-0007p). |
| Comment from the depositor | |
| Terms and conditions | 1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
10s
|
| Tissue |
skin
|
| Disease name |
Muscular dystrophy, Duchenne type
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
SeV18+HS-OCT3/4/TSΔF, SeV18+HS-SOX2/TSΔF, SeV18+HS-KLF4/TSΔF, SeV18(HNL)c-MYCQC/TSΔF
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
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|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-5 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
|
37
℃
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| CO2 concentration |
|
3
%
|
| Feeder cells |
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MEF
|
| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10^(5) cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
|
Vitrification
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| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
2
|
| User's Publication |
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10738
Long C, Li H, Tiburcy M, Rodriguez-Caycedo C, Kyrychenko V, Zhou H, Zhang Y, Min YL, Shelton JM, Mammen PPA, Liaw NY, Zimmermann WH, Bassel-Duby R, Schneider JW, Olson EN.
Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing.
Sci Adv
2018
4:eaap9004
PubMed ID: 29404407
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10265
Zhang Y, Long C, Li H, McAnally JR, Baskin KK, Shelton JM, Bassel-Duby R, Olson EN.
CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice.
Sci Adv
2017
3:e1602814
PubMed ID: 28439558
|
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SDS_DAP(MEM)_Japanese.pdf