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Cell No. : Cell Name
HPS0141 :  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : Amyotrophic lateral sclerosis (ALS), Familial type. HPS0140 and HPS0142 were derived from the same patient. iPS cells were created using the Sendai virus vector. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 3) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the APPROVER. 4) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   Approval Form(C-0057.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 50s
Tissue skin
Disease name amyotrophic lateral sclerosis (ALS)
Classification iPS
Recombinant recombinant
Exogene SeV18+HS-OCT3/4/TSΔF, SeV18+HS-SOX2/TSΔF, SeV18+HS-KLF4/TSΔF, SeV18(HNL)c-MYCQC/TSΔF
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_Y978
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10 5 cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma/Achoreplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 0


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