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Cell No. : Cell Name
HPS0082 : iPWS5  update : 2022/01/20
CommentDisease specific iPS cell line derived from a patient : Prader Willi syndrome (RCB1560 PWS-Yamaguchi). del(15) was found in lymphocytes of the patient. iPS cells were created using the Sendai virus vector.
Comment from the depositor
Terms and conditions1) The iPS cells created using the Sendai virus vector are now available must be observed when using SeV-iPS cells. 2) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 4) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Era, Takumi
Originator Era, Takumi
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 20s
Tissue skin
Disease name Prader-Willi syndrome
Classification iPS
Recombinant recombinant
Exogene SeV18+HS-OCT3/4/TSΔF, SeV18+HS-SOX2/TSΔF, SeV18+HS-KLF4/TSΔF, SeV18(HNL)c-MYCQC/TSΔF
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T947
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 2


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Reference

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User's Publication
15521  Soeda S, Saito R, Fujii A, Tojo S, Tokumura Y, Taniura H.  Abnormal DNA methylation in pluripotent stem cells from a patient with Prader-Willi syndrome results in neuronal differentiation defects  Stem Cell Res  2021  53:102351  PubMed ID: 33895503
4051  Soeda S, Saito R, Fujita N, Fukuta K, Taniura H.  Neuronal differentiation defects in induced pluripotent stem cells derived from a Prader-Willi syndrome patient  Neurosci Lett  2019  703:162-167  PubMed ID: 30902571



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