CELL SEARCH SYSTEM -CELL BANK- (RIKEN BRC) [HPS0077 : 454E2]

細胞番号 : 細胞名
HPS0077 : 454E2 update : 2024/03/29

細胞特性(Comment:英)

Stopped distribution temporarily. Human iPS cell line. Cryopreserved by vitrification method. Application consideration. Please see CiRA Foundation, if you are a researcher at a for-profit organization.

細胞特性(日)

一時的に提供を中止しています。ヒト人工多能性幹（iPS）細胞株。エピソーマルベクター (pCXLE)によりヒト6因子（Oct3/4, Sox2,Klf4,L-Myc,Lin28,p53 shRNA）を導入して樹立。導入遺伝子は残っていない。ガラス化法（Vitrification法）保存。培養開始にあたっての注意。営利機関は、公益財団法人京都大学iPS細胞研究財団から配布。

細胞特性(寄託者記述:英)

細胞特性(寄託者記述:日)

使用条件(英)

1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA.

使用条件(日)

1) 利用者は学術機関に所属している者に限る。2) 別紙1を提供同意書に添付すること。

備考(英)

備考(日)

提供申込書類(英)

Order Form(C-0042.pdf) MTA(C-0007.pdf)

Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).

提供申込書類(日)

依頼書C-0041.pdf 同意書(非営利学術目的)C-0003.pdf

提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。

提供手数料

手数料とお支払いについてはこちらをご覧ください。

細胞基本情報

寄託者

https://www.cira.kyoto-u.ac.jp/

樹立者

Yamanaka, Shinya

寄託日

2011

動物種

_human < Mammals

属名

Homo

種名

sapiens

人種

Japanese

性別

Female

齢

16 years

採取組織

dental pulp

細胞分類

iPS

遺伝子改変

recombinant

外来遺伝子

pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA

細胞寿命

infinite

細胞形態

ES-like

Cellosaurus(Expasy)

CVCL_T791

細胞培養・検査情報

寄託時情報

ロット情報

培地・試薬情報

培地・試薬一覧はこちらをご覧ください。

培養形態

Adherent cells

培地

Primate ES Cell Medium + 4ng/ml human basic FGF

抗生物質

Free

継代方法

0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)

培養容器のコーティング

0.1% gelatin coated dish

継代密度

1 : 3-5 split

継代・培地交換頻度

Subculture : once/3-5 days, Medium Renewal : every day

培養最適温度

37 ℃

二酸化炭素濃度

5 %

フィーダー細胞

SNL 76/7

フィーダー細胞の処理方法

X-rays 5000R (or MMC)

フィーダー細胞の播種数

1.5-2.5 x 10⁶ cells/100 mm dish

凍結培地

DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol

凍結方法

Vitrification

マイコプラズマ/アコレプラズマ

(-)

染色体検査

46,XX[20] at passage No. 24

個体識別検査

画像情報

寄託時情報

ロット情報

その他の細胞情報

寄託時情報

ロット情報

SDS_DAP(MEM)_Japanese.pdf

文献情報

Reference(英)

2件

Reference(日)

0件

利用者成果(英)

45件

利用者成果(日)

0件

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Reference(英)

21869 Hitomi Aoki Generation of iPSCs Using Sendai Virus Vectors Methods Mol Biol 2024 2794:121-140. PubMed ID: 38630225 DOI: 10.1007/978-1-0716-3810-1_11

3676 Okita K1, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. A more efficient method to generate integration-free human iPS cells. Nat Methods 2011 8(5):409-12 PubMed ID: 21460823 DOI: 10.1038/nmeth.1591

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Reference(日)

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利用者成果(英)

21607 Sung TC, Chen YH, Wang T, Qian L, Chao WH, Liu J, Pang J, Ling QD, Lee HH, Higuchi A. Design of dual peptide-conjugated hydrogels for proliferation and differentiation of human pluripotent stem cells. Mater Today Bio 2024 25:100969 PubMed ID: 38318478 DOI: 10.1016/j.mtbio.2024.100969

19714 Wang T, Liu Q, Chang YT, Liu J, Yu T, Maitiruze K, Ban LK, Sung TC, Subbiah SK, Renuka RR, Jen SH, Lee HH, Higuchi A. Designed peptide-grafted hydrogels for human pluripotent stem cell culture and differentiation J Mater Chem B 2023 11(7):1434-1444 PubMed ID: 36541288 DOI: 10.1039/d2tb02521c

21497 Koji Nakade, Satomi Tsukamoto, Kenichi Nakashima, Yuri An, Iori Sato, Jingyue Li, Yuzuno Shimoda, Yasuko Hemmi, Yoshihiro Miwa, Yohei Hayashi Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system Cell Reports Methods 2023 PubMed ID: 38086384 DOI: 10.1016/j.crmeth.2023.100662

21513 Liu Q, Liu J, Guo M, Sung TC, Wang T, Yu T, Tian Z, Fan G, Wu W, Higuchi A. Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration model. J Transl Med 2023 21(1):910 PubMed ID: 38098048 DOI: 10.1186/s12967-023-04785-1

16702 Song D, Takahashi G, Zheng YW, Matsuo-Takasaki M, Li J, Takami M, An Y, Hemmi Y, Miharada N, Fujioka T, Noguchi M, Nakajima T, Saito MK, Nakamura Y, Oda T, Miyaoka Y, Hayashi Y. Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes Hum Mol Genet 2022 Apr 7;ddac080 PubMed ID: 35388883 DOI: 10.1093/hmg/ddac080

20945 Kanzaki Y, Fujita H, Sato K, Hosokawa M, Matsumae H, Morizane Y, Ohuchi H. Protrusion of KCNJ13 Gene Knockout Retinal Pigment Epithelium Due to Oxidative Stress-Induced Cell Death. Invest Ophthalmol Vis Sci 2022 63(12):29 PubMed ID: 36413373 DOI: 10.1167/iovs.63.12.29

13461 Sung TC, Jiang YP, Hsu JY, Ling QD, Chen H, Kumar SS, Chang Y, Hsu ST, Ye Q, Higuchi A. Transient characteristics of universal cells on human-induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources Cell Prolif 2021 e12995 PubMed ID: 33522648 DOI: 10.1111/cpr.12995

13499 Homma K, Toda E, Osada H, Nagai N, Era T, Tsubota K, Okano H, Ozawa Y. Taurine rescues mitochondria-related metabolic impairments in the patient-derived induced pluripotent stem cells and epithelial-mesenchymal transition in the retinal pigment epithelium Redox Biol 2021 41:101921 PubMed ID: 33706170 DOI: 10.1016/j.redox.2021.101921

20308 Danoy M, Jellali R, Tauran Y, Bruce J, Leduc M, Gilard F, Gakière B, Scheidecker B, Kido T, Miyajima A, Soncin F, Sakai Y, Leclerc E. Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells. Differentiation 2021 120:28-35 PubMed ID: 34229994 DOI: 10.1016/j.diff.2021.06.001

15509 Danoy M, Tauran Y, Poulain S, Jellali R, Bruce J, Leduc M, Le Gall M, Koui Y, Arakawa H, Gilard F, Gakiere B, Kato Y, Plessy C, Kido T, Miyajima A, Sakai Y, Leclerc E. Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment APL Bioeng 2021 5(2):026104 PubMed ID: 34027283 DOI: 10.1063/5.0041227

15523 Abe T, Uosaki H, Shibata H, Hara H, Sarentonglaga B, Nagao Y, Hanazono Y. Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells Exp Hematol 2021 95:46-57.e8 PubMed ID: 33395577 DOI: 10.1016/j.exphem.2020.12.006

15527 Kimura S, Morizane Y, Toshima S, Shiode Y, Doi S, Takahashi K, Matoba R, Kanzaki Y, Shiraga F. Cytotoxic effects of alteplase, a recombinant tissue plasminogen activator, on human retinal pigment epithelial cells Jpn J Ophthalmol 2021 65(5):731-739 PubMed ID: 34117982 DOI: 10.1007/s10384-021-00848-2

20491 Tadokoro S, Tokuyama-Toda R, Tatehara S, Ide S, Umeki H, Miyoshi K, Noma T, Satomura K. A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections. Cells 2021 10(11) PubMed ID: 34831050 DOI: 10.3390/cells10112827

4117 Csobonyeiova M, Polak S, Danisovic L. Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling. Exp. Clin. Endocrinol. Diabetes 2020 PubMed ID: 30114722 DOI: 10.1055/a-0661-5873

6879 Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H. Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells. Cell Rep 2020 30(1):18-24 PubMed ID: 31914385 DOI: 10.1016/j.celrep.2019.12.009

6969 Aoyama J, Homma K, Tanabe N, Usui S, Miyagi Y, Matsuura K, Kaneda M, Nitta T. Spatiotemporal imaging documented the maturation of the cardiomyocytes from human induced pluripotent stem cells. J Thorac Cardiovasc Surg 2020 159(6):2260-2271.e7 PubMed ID: 31409490 DOI: 10.1016/j.jtcvs.2019.06.060

12042 Ninomiya H, Intoh A, Ishimine H, Onuma Y, Ito Y, Michiue T, Tazaki A, Kato M. Application of a human mesoderm tissue elongation system in vitro derived from human induced pluripotent stem cells to risk assessment for teratogenic chemicals. Chemosphere 2020 PubMed ID: 32092576 DOI: 10.1016/j.chemosphere.2020.126124

13000 Sung TC, Su HC, Ling QD, Kumar SS, Chang Y, Hsu ST, Higuchi A. Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface. Biomaterials 2020 PubMed ID: 32450407 DOI: 10.1016/j.biomaterials.2020.120060

13214 Higuchi A, Hirad AH, Kumar SS, Munusamy MA, Alarfaj AA. Thermoresponsive surfaces designed for the proliferation and differentiation of human pluripotent stem cells. Acta Biomater 2020 PubMed ID: 32911107 DOI: 10.1016/j.actbio.2020.09.010

13488 Danoy M, Poulain S, Koui Y, Tauran Y, Scheidecker B, Kido T, Miyajima A, Sakai Y, Plessy C, Leclerc E. Transcriptome profiling of hiPSC-derived LSECs with nanoCAGE Mol Omics 2020 16(2):138-146 PubMed ID: 31989141 DOI: 10.1039/c9mo00135b

13893 Kanzaki Y, Fujita H, Sato K, Hosokawa M, Matsumae H, Shiraga F, Morizane Y, Ohuchi H. KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells Invest Ophthalmol Vis Sci 2020 61(5):38 PubMed ID: 32437550 DOI: 10.1167/iovs.61.5.38

11745 Sung TC, Yang JS, Yeh CC, Liu YC, Jiang YP, Lu MW, Ling QD, Kumar SS, Chang Y, Umezawa A, Chen H, Higuchi A. The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells. Biomaterials 2019 PubMed ID: 31419657 DOI: 10.1016/j.biomaterials.2019.119411

11798 Sugawara Y, Hamada K, Yamada Y, Kumai J, Kanagawa M, Kobayashi K, Toda T, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Kikkawa Y. Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment. Sci Rep 2019 PubMed ID: 31506597 DOI: 10.1038/s41598-019-49669-x

6894 Chen LH , Sung TC , Lee HH , Higuchi A , Su HC , Lin KJ , Huang YR , Ling QD , Kumar SS , Alarfaj AA , Munusamy MA , Nasu M , Chen DC , Hsu ST , Chang Y , Lee KF , Wang HC , Umezawa A . Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels. Biomater Sci 2019 7(10):4345-4362 PubMed ID: 31411209 DOI: 10.1039/c9bm00418a

12369 Kamao H, Miki A, Kiryu J. Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activat J Ophthalmol 2019 2019:7189241 PubMed ID: 31016039 DOI: 10.1155/2019/7189241

14057 Sung TC, Liu CH, Huang WL, Lee YC, Kumar SS, Chang Y, Ling QD, Hsu ST, Higuchi A. Efficient differentiation of human ES and iPS cells into cardiomyocytes on biomaterials under xeno-free conditions Biomater Sci 2019 7(12):5467-5481 PubMed ID: 31656967 DOI: 10.1039/c9bm00817a

10713 Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG. Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration. Sci Rep 2018 8:461 PubMed ID: 29323137 DOI: 10.1038/s41598-017-17634-1

11234 Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y. Tumorigenicity-associated characteristics of human iPS cell lines. PLoS ONE 2018 PubMed ID: 30286143 DOI: 10.1371/journal.pone.0205022

5773 Kim MH, Matsubara Y, Fujinaga Y, Kino-Oka M. A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin. Biotechnol J 2018 13(2) PubMed ID: 29027750 DOI: 10.1002/biot.201700384

7022 Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nat. Methods 2017 14:963-966 PubMed ID: 28892089 DOI: 10.1038/nmeth.4430

7026 Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A. An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells. Stem Cell Reports 2017 9:490-498 PubMed ID: 28757162 DOI: 10.1016/j.stemcr.2017.06.010

10064 Lin HR, Heish CW, Liu CH, Muduli S, Li HF, Higuchi A, Kumar SS, Alarfaj AA, Munusamy MA, Hsu ST, Chen DC, Benelli G, Murugan K, Cheng NC, Wang HC, Wu GJ. Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods. Sci Rep 2017 7:40069 PubMed ID: 28071738 DOI: 10.1038/srep40069

10071 Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M. Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation. Invest. Ophthalmol. Vis. Sci. 2017 58:211-220 PubMed ID: 28114582 DOI: 10.1167/iovs.16-19778

10093 Homma K, Usui S, Kaneda M. Knock-in strategy at 3'-end of Crx gene by CRISPR/Cas9 system shows the gene expression profiles during human photoreceptor differentiation. Genes Cells 2017 22:250-264 PubMed ID: 28124402 DOI: 10.1111/gtc.12472

10216 Chen YM, Chen LH, Li MP, Li HF, Higuchi A, Kumar SS, Ling QD, Alarfaj AA, Munusamy MA, Chang Y, Benelli G, Murugan K, Umezawa A. Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs. Sci Rep 2017 7:45146 PubMed ID: 28332572 DOI: 10.1038/srep45146

10373 Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M. Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302. Stem Cell Res 2017 23:13-19 PubMed ID: 28666145 DOI: 10.1016/j.scr.2017.06.011

4551 Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K. Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nat Protoc 2017 12(4):683-696 PubMed ID: 28253236 DOI: 10.1038/nprot.2017.007

7080 Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K. Co-ordinated ocular development from human iPS cells and recovery of corneal function. Nature 2016 531:376-80 PubMed ID: 26958835 DOI: 10.1038/nature17000

9686 Konagaya S, Iwata H. Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study. Biochim. Biophys. Acta 2016 1860:2008-16 PubMed ID: 27180174 DOI: 10.1016/j.bbagen.2016.05.012

7112 Higuchi A, Kao SH, Ling QD, Chen YM, Li HF, Alarfaj AA, Munusamy MA, Murugan K, Chang SC, Lee HC, Hsu ST, Kumar SS, Umezawa A. Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Sci Rep 2015 5:18136 PubMed ID: 26656754 DOI: 10.1038/srep18136

7213 Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A. CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells. Stem Cell Reports 2015 5:508-15 PubMed ID: 26365514 DOI: 10.1016/j.stemcr.2015.08.008

5790 Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H. Long-term maintenance of human induced pluripotent stem cells by automated cell culture system. Sci Rep 2015 5:16647 PubMed ID: 26573336 DOI: 10.1038/srep16647

5792 Nagaoka M, Kobayashi M, Kawai C, Mallanna SK, Duncan SA. Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells. PLoS One 2015 10(8):e0136350 PubMed ID: 26308339 DOI: 10.1371/journal.pone.0136350

15246 Higuchi A, Wang CT, Ling QD, Lee HH, Kumar SS, Chang Y, Alarfaj AA, Munusamy MA, Hsu ST, Wu GJ, Umezawa A. A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues Sci Rep 2015 5:10217 PubMed ID: 25970301 DOI: 10.1038/srep10217

5787 Kamao H, Mandai M, Wakamiya S, Ishida J, Goto K, Ono T, Suda T, Takahashi M, Kiryu J. Objective evaluation of the degree of pigmentation in human induced pluripotent stem cell-derived RPE. Invest Ophthalmol Vis Sci 2014 55(12):8309-18. PubMed ID: 25389202 DOI: 10.1167/iovs.14-14694

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