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Cell No. : Cell Name
HPS0077 : 454E2  update : 2021/04/26
CommentHuman iPS cell line. Application consideration. Order Form (C-0042, C-0034). Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Order Form Order Form(C-0042.pdf)   MTA(C-0034.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2011
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 16 years
Tissue dental pulp
Classification iPS
Recombinant recombinant
Exogene pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Chromosome mode 46XX(20)
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 1
User's Publication 31

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3676  Okita K1, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.  A more efficient method to generate integration-free human iPS cells.  Nat Methods  2011  8(5):409-12  PubMed ID: 21460823

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User's Publication
13461  Sung TC, Jiang YP, Hsu JY, Ling QD, Chen H, Kumar SS, Chang Y, Hsu ST, Ye Q, Higuchi A.  Transient characteristics of universal cells on human-induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources  Cell Prolif  2021  e12995  PubMed ID: 33522648
6879  Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H.  Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells.  Cell Rep  2020  30(1):18-24  PubMed ID: 31914385
6969  Aoyama J, Homma K, Tanabe N, Usui S, Miyagi Y, Matsuura K, Kaneda M, Nitta T.  Spatiotemporal imaging documented the maturation of the cardiomyocytes from human induced pluripotent stem cells.  J Thorac Cardiovasc Surg  2020  159(6):2260-2271.e7  PubMed ID: 31409490
12042  Ninomiya H, Intoh A, Ishimine H, Onuma Y, Ito Y, Michiue T, Tazaki A, Kato M.  Application of a human mesoderm tissue elongation system in vitro derived from human induced pluripotent stem cells to risk assessment for teratogenic chemicals.  Chemosphere  2020    PubMed ID: 32092576
13000  Sung TC, Su HC, Ling QD, Kumar SS, Chang Y, Hsu ST, Higuchi A.  Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface.  Biomaterials  2020    PubMed ID: 32450407
13214  Higuchi A, Hirad AH, Kumar SS, Munusamy MA, Alarfaj AA.  Thermoresponsive surfaces designed for the proliferation and differentiation of human pluripotent stem cells.  Acta Biomater  2020    PubMed ID: 32911107
13488  Danoy M, Poulain S, Koui Y, Tauran Y, Scheidecker B, Kido T, Miyajima A, Sakai Y, Plessy C, Leclerc E.  Transcriptome profiling of hiPSC-derived LSECs with nanoCAGE  Mol Omics  2020  16(2):138-146  PubMed ID: 31989141
13893  Kanzaki Y, Fujita H, Sato K, Hosokawa M, Matsumae H, Shiraga F, Morizane Y, Ohuchi H.  KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells  Invest Ophthalmol Vis Sci  2020  61(5):38  PubMed ID: 32437550
11745  Sung TC, Yang JS, Yeh CC, Liu YC, Jiang YP, Lu MW, Ling QD, Kumar SS, Chang Y, Umezawa A, Chen H, Higuchi A.  The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells.  Biomaterials  2019    PubMed ID: 31419657
11798  Sugawara Y, Hamada K, Yamada Y, Kumai J, Kanagawa M, Kobayashi K, Toda T, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Kikkawa Y.  Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment.  Sci Rep  2019    PubMed ID: 31506597
6894  Chen LH , Sung TC , Lee HH , Higuchi A , Su HC , Lin KJ , Huang YR , Ling QD , Kumar SS , Alarfaj AA , Munusamy MA , Nasu M , Chen DC , Hsu ST , Chang Y , Lee KF , Wang HC , Umezawa A .  Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels.  Biomater Sci  2019  7(10):4345-4362  PubMed ID: 31411209
12369  Kamao H, Miki A, Kiryu J.  Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activat  J Ophthalmol  2019  2019:7189241  PubMed ID: 31016039
10713  Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG.  Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.  Sci Rep  2018  8:461  PubMed ID: 29323137
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2018    PubMed ID: 30114722
5773  Kim MH, Matsubara Y, Fujinaga Y, Kino-Oka M.  A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin.  Biotechnol J  2018  13(2)  PubMed ID: 29027750
7022  Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.  CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.  Nat. Methods  2017  14:963-966  PubMed ID: 28892089
7026  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162
10064  Lin HR, Heish CW, Liu CH, Muduli S, Li HF, Higuchi A, Kumar SS, Alarfaj AA, Munusamy MA, Hsu ST, Chen DC, Benelli G, Murugan K, Cheng NC, Wang HC, Wu GJ.  Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods.  Sci Rep  2017  7:40069  PubMed ID: 28071738
10071  Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582
10093  Homma K, Usui S, Kaneda M.  Knock-in strategy at 3'-end of Crx gene by CRISPR/Cas9 system shows the gene expression profiles during human photoreceptor differentiation.  Genes Cells  2017  22:250-264  PubMed ID: 28124402
10216  Chen YM, Chen LH, Li MP, Li HF, Higuchi A, Kumar SS, Ling QD, Alarfaj AA, Munusamy MA, Chang Y, Benelli G, Murugan K, Umezawa A.  Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.  Sci Rep  2017  7:45146  PubMed ID: 28332572
10373  Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.  Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.  Stem Cell Res  2017  23:13-19  PubMed ID: 28666145
4551  Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K.  Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells.  Nat Protoc  2017  12(4):683-696  PubMed ID: 28253236
7080  Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835
9686  Konagaya S, Iwata H.  Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study.  Biochim. Biophys. Acta  2016  1860:2008-16  PubMed ID: 27180174
7112  Higuchi A, Kao SH, Ling QD, Chen YM, Li HF, Alarfaj AA, Munusamy MA, Murugan K, Chang SC, Lee HC, Hsu ST, Kumar SS, Umezawa A.  Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.  Sci Rep  2015  5:18136  PubMed ID: 26656754
7213  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514
5790  Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.  Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.  Sci Rep  2015  5:16647  PubMed ID: 26573336
5792  Nagaoka M, Kobayashi M, Kawai C, Mallanna SK, Duncan SA.  Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.  PLoS One  2015  10(8):e0136350  PubMed ID: 26308339
5787  Kamao H, Mandai M, Wakamiya S, Ishida J, Goto K, Ono T, Suda T, Takahashi M, Kiryu J.  Objective evaluation of the degree of pigmentation in human induced pluripotent stem cell-derived RPE.  Invest Ophthalmol Vis Sci  2014  55(12):8309-18.  PubMed ID: 25389202

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