Cell No. : Cell Name HPS0077 : 454E2  update : 2025/01/16
Comment			Stopped distribution temporarily. Human iPS cell line. Cryopreserved by vitrification method. Application consideration. Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions			1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA.
Remarks
Order Form			Order Form(C-0042.pdf)   MTA(C-0007.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	https://www.cira.kyoto-u.ac.jp/
		Originator	Yamanaka, Shinya
		Year of deposit	2011
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	16 years
		Tissue	dental pulp
		Classification	iPS
		Recombinant	recombinant
		Exogene	pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_T791
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		Primate ES Cell Medium + 4ng/ml human basic FGF
		Antibiotics		Free
		Passage method		0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage ratio		1 : 3-5 split
		SC frequency		Subculture : once/3-5 days, Medium Renewal : every day
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		SNL 76/7
		Feeder treatment		X-rays 5000R (or MMC)
		Feeder seed cells		1.5-2.5 x 10 6 cells/100 mm dish
		Freeze medium		DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
		Freezing method		Vitrification
		Mycoplasma/Acholeplasma		(-)
		Chromosome mode		46,XX[20] at passage No. 24
		STR(human)		OK
Images			deposit info	lot info
Relational File			deposit info	lot info
				SDS_DAP(MEM)_Japanese.pdf
Reference information		Reference	2
		User's Publication	50

Top

Reference

21869  Hitomi Aoki  Generation of iPSCs Using Sendai Virus Vectors  Methods Mol Biol  2024  2794:121-140.  PubMed ID: 38630225   DOI: 10.1007/978-1-0716-3810-1_11

3676  Okita K1, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.  A more efficient method to generate integration-free human iPS cells.  Nat Methods  2011  8(5):409-12  PubMed ID: 21460823   DOI: 10.1038/nmeth.1591

Top

User's Publication

22566  Sung TC, Pan ZX, Wang T, Lin HY, Chang CL, Hung LC, Subbiah SK, Renuka RR, Chou SJ, Chiou SH, Joanna I, Lee HH, Wu GJ, Higuchi A.  Material surface conjugated with fibroblast growth factor-2 for pluripotent stem cell culture and differentiation.  Regen Biomater  2025  12:rbaf003  PubMed ID: 39967781   DOI: 10.1093/rb/rbaf003

22744  Guillaud L, Garanzini A, Zakhia S, De la Fuente S, Dimitrov D, Boerner S, Terenzio M.  Loss of intracellular ATP affects axoplasmic viscosity and pathological protein aggregation in mammalian neurons.  Sci Adv  2025  11(17):eadq6077  PubMed ID: 40267187   DOI: 10.1126/sciadv.adq6077

22988  Sung TC, Chao WH, Tian Z, Chen J, Jiang C, Gong J, Pan J, Wang T, Ling QD, Higuchi A.  Conjugation of peptides to a dendrimer surface to promote the proliferation and differentiation of human pluripotent stem cells into cardiomyocytes and retinal pigment epithelium.  Biomaterials  2025  324:123506  PubMed ID: 40532655   DOI: 10.1016/j.biomaterials.2025.123506

21607  Sung TC, Chen YH, Wang T, Qian L, Chao WH, Liu J, Pang J, Ling QD, Lee HH, Higuchi A.  Design of dual peptide-conjugated hydrogels for proliferation and differentiation of human pluripotent stem cells.  Mater Today Bio  2024  25:100969  PubMed ID: 38318478   DOI: 10.1016/j.mtbio.2024.100969

22171  Matsuo-Takasaki M, Kambayashi S, Hemmi Y, Wakabayashi T, Shimizu T, An Y, Ito H, Takeuchi K, Ibuki M, Kawashima T, Masayasu R, Suzuki M, Kawai Y, Umekage M, Kato TM, Noguchi M, Nakade K, Nakamura Y, Nakaishi T, Nishishita N, Tsukahara M, Hayashi Y.  Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation.  Elife  2024  12  PubMed ID: 39529479   DOI: 10.7554/eLife.89724

19714  Wang T, Liu Q, Chang YT, Liu J, Yu T, Maitiruze K, Ban LK, Sung TC, Subbiah SK, Renuka RR, Jen SH, Lee HH, Higuchi A.  Designed peptide-grafted hydrogels for human pluripotent stem cell culture and differentiation  J Mater Chem B  2023  11(7):1434-1444  PubMed ID: 36541288   DOI: 10.1039/d2tb02521c

21497  Koji Nakade, Satomi Tsukamoto, Kenichi Nakashima, Yuri An, Iori Sato, Jingyue Li, Yuzuno Shimoda, Yasuko Hemmi, Yoshihiro Miwa, Yohei Hayashi  Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system  Cell Reports Methods  2023    PubMed ID: 38086384   DOI: 10.1016/j.crmeth.2023.100662

21513  Liu Q, Liu J, Guo M, Sung TC, Wang T, Yu T, Tian Z, Fan G, Wu W, Higuchi A.  Comparison of retinal degeneration treatment with four types of different mesenchymal stem cells, human induced pluripotent stem cells and RPE cells in a rat retinal degeneration model.  J Transl Med  2023  21(1):910  PubMed ID: 38098048   DOI: 10.1186/s12967-023-04785-1

22620  Danoy M, Poulain S, Jellali R, Scheidecker B, Tauran Y, Leduc M, Bruce J, Kim SH, Kido T, Miyajima A, Sakai Y, Leclerc E.  Transcriptomic and proteomic studies suggest the establishment of advanced zonation-like profiles in human-induced pluripotent stem cell-derived liver sinusoidal endothelial cells and carboxypeptidase M-positive liver progenitor cells cocultured in a fluidic microenvironment  Hepatol Res  2023  53(7):661-674  PubMed ID: 36866738   DOI: 10.1111/hepr.13893

16702  Song D, Takahashi G, Zheng YW, Matsuo-Takasaki M, Li J, Takami M, An Y, Hemmi Y, Miharada N, Fujioka T, Noguchi M, Nakajima T, Saito MK, Nakamura Y, Oda T, Miyaoka Y, Hayashi Y.  Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes  Hum Mol Genet  2022  Apr 7;ddac080  PubMed ID: 35388883   DOI: 10.1093/hmg/ddac080

20945  Kanzaki Y, Fujita H, Sato K, Hosokawa M, Matsumae H, Morizane Y, Ohuchi H.  Protrusion of KCNJ13 Gene Knockout Retinal Pigment Epithelium Due to Oxidative Stress-Induced Cell Death.  Invest Ophthalmol Vis Sci  2022  63(12):29  PubMed ID: 36413373   DOI: 10.1167/iovs.63.12.29

13461  Sung TC, Jiang YP, Hsu JY, Ling QD, Chen H, Kumar SS, Chang Y, Hsu ST, Ye Q, Higuchi A.  Transient characteristics of universal cells on human-induced pluripotent stem cells and their differentiated cells derived from foetal stem cells with mixed donor sources  Cell Prolif  2021  e12995  PubMed ID: 33522648   DOI: 10.1111/cpr.12995

13499  Homma K, Toda E, Osada H, Nagai N, Era T, Tsubota K, Okano H, Ozawa Y.  Taurine rescues mitochondria-related metabolic impairments in the patient-derived induced pluripotent stem cells and epithelial-mesenchymal transition in the retinal pigment epithelium  Redox Biol  2021  41:101921  PubMed ID: 33706170   DOI: 10.1016/j.redox.2021.101921

20308  Danoy M, Jellali R, Tauran Y, Bruce J, Leduc M, Gilard F, Gakière B, Scheidecker B, Kido T, Miyajima A, Soncin F, Sakai Y, Leclerc E.  Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells.  Differentiation  2021  120:28-35  PubMed ID: 34229994   DOI: 10.1016/j.diff.2021.06.001

15509  Danoy M, Tauran Y, Poulain S, Jellali R, Bruce J, Leduc M, Le Gall M, Koui Y, Arakawa H, Gilard F, Gakiere B, Kato Y, Plessy C, Kido T, Miyajima A, Sakai Y, Leclerc E.  Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment  APL Bioeng  2021  5(2):026104  PubMed ID: 34027283   DOI: 10.1063/5.0041227

15523  Abe T, Uosaki H, Shibata H, Hara H, Sarentonglaga B, Nagao Y, Hanazono Y.  Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells  Exp Hematol   2021  95:46-57.e8  PubMed ID: 33395577   DOI: 10.1016/j.exphem.2020.12.006

15527  Kimura S, Morizane Y, Toshima S, Shiode Y, Doi S, Takahashi K, Matoba R, Kanzaki Y, Shiraga F.  Cytotoxic effects of alteplase, a recombinant tissue plasminogen activator, on human retinal pigment epithelial cells  Jpn J Ophthalmol  2021  65(5):731-739  PubMed ID: 34117982   DOI: 10.1007/s10384-021-00848-2

20491  Tadokoro S, Tokuyama-Toda R, Tatehara S, Ide S, Umeki H, Miyoshi K, Noma T, Satomura K.  A New Induction Method for the Controlled Differentiation of Human-Induced Pluripotent Stem Cells Using Frozen Sections.  Cells  2021  10(11)  PubMed ID: 34831050   DOI: 10.3390/cells10112827

4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2020    PubMed ID: 30114722   DOI: 10.1055/a-0661-5873

6879  Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H.  Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells.  Cell Rep  2020  30(1):18-24  PubMed ID: 31914385   DOI: 10.1016/j.celrep.2019.12.009

6969  Aoyama J, Homma K, Tanabe N, Usui S, Miyagi Y, Matsuura K, Kaneda M, Nitta T.  Spatiotemporal imaging documented the maturation of the cardiomyocytes from human induced pluripotent stem cells.  J Thorac Cardiovasc Surg  2020  159(6):2260-2271.e7  PubMed ID: 31409490   DOI: 10.1016/j.jtcvs.2019.06.060

12042  Ninomiya H, Intoh A, Ishimine H, Onuma Y, Ito Y, Michiue T, Tazaki A, Kato M.  Application of a human mesoderm tissue elongation system in vitro derived from human induced pluripotent stem cells to risk assessment for teratogenic chemicals.  Chemosphere  2020    PubMed ID: 32092576   DOI: 10.1016/j.chemosphere.2020.126124

13000  Sung TC, Su HC, Ling QD, Kumar SS, Chang Y, Hsu ST, Higuchi A.  Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface.  Biomaterials  2020    PubMed ID: 32450407   DOI: 10.1016/j.biomaterials.2020.120060

13214  Higuchi A, Hirad AH, Kumar SS, Munusamy MA, Alarfaj AA.  Thermoresponsive surfaces designed for the proliferation and differentiation of human pluripotent stem cells.  Acta Biomater  2020    PubMed ID: 32911107   DOI: 10.1016/j.actbio.2020.09.010

13488  Danoy M, Poulain S, Koui Y, Tauran Y, Scheidecker B, Kido T, Miyajima A, Sakai Y, Plessy C, Leclerc E.  Transcriptome profiling of hiPSC-derived LSECs with nanoCAGE  Mol Omics  2020  16(2):138-146  PubMed ID: 31989141   DOI: 10.1039/c9mo00135b

13893  Kanzaki Y, Fujita H, Sato K, Hosokawa M, Matsumae H, Shiraga F, Morizane Y, Ohuchi H.  KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells  Invest Ophthalmol Vis Sci  2020  61(5):38  PubMed ID: 32437550   DOI: 10.1167/iovs.61.5.38

11745  Sung TC, Yang JS, Yeh CC, Liu YC, Jiang YP, Lu MW, Ling QD, Kumar SS, Chang Y, Umezawa A, Chen H, Higuchi A.  The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells.  Biomaterials  2019    PubMed ID: 31419657   DOI: 10.1016/j.biomaterials.2019.119411

11798  Sugawara Y, Hamada K, Yamada Y, Kumai J, Kanagawa M, Kobayashi K, Toda T, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Kikkawa Y.  Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment.  Sci Rep  2019    PubMed ID: 31506597   DOI: 10.1038/s41598-019-49669-x

6894  Chen LH , Sung TC , Lee HH , Higuchi A , Su HC , Lin KJ , Huang YR , Ling QD , Kumar SS , Alarfaj AA , Munusamy MA , Nasu M , Chen DC , Hsu ST , Chang Y , Lee KF , Wang HC , Umezawa A .  Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels.  Biomater Sci  2019  7(10):4345-4362  PubMed ID: 31411209   DOI: 10.1039/c9bm00418a

12369  Kamao H, Miki A, Kiryu J.  Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activat  J Ophthalmol  2019  2019:7189241  PubMed ID: 31016039   DOI: 10.1155/2019/7189241

14057  Sung TC, Liu CH, Huang WL, Lee YC, Kumar SS, Chang Y, Ling QD, Hsu ST, Higuchi A.  Efficient differentiation of human ES and iPS cells into cardiomyocytes on biomaterials under xeno-free conditions  Biomater Sci  2019  7(12):5467-5481  PubMed ID: 31656967   DOI: 10.1039/c9bm00817a

10713  Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG.  Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.  Sci Rep  2018  8:461  PubMed ID: 29323137   DOI: 10.1038/s41598-017-17634-1

11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143   DOI: 10.1371/journal.pone.0205022

5773  Kim MH, Matsubara Y, Fujinaga Y, Kino-Oka M.  A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin.  Biotechnol J  2018  13(2)  PubMed ID: 29027750   DOI: 10.1002/biot.201700384

7022  Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.  CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.  Nat. Methods  2017  14:963-966  PubMed ID: 28892089   DOI: 10.1038/nmeth.4430

7026  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162   DOI: 10.1016/j.stemcr.2017.06.010

10064  Lin HR, Heish CW, Liu CH, Muduli S, Li HF, Higuchi A, Kumar SS, Alarfaj AA, Munusamy MA, Hsu ST, Chen DC, Benelli G, Murugan K, Cheng NC, Wang HC, Wu GJ.  Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods.  Sci Rep  2017  7:40069  PubMed ID: 28071738   DOI: 10.1038/srep40069

10071  Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582   DOI: 10.1167/iovs.16-19778

10093  Homma K, Usui S, Kaneda M.  Knock-in strategy at 3'-end of Crx gene by CRISPR/Cas9 system shows the gene expression profiles during human photoreceptor differentiation.  Genes Cells  2017  22:250-264  PubMed ID: 28124402   DOI: 10.1111/gtc.12472

10216  Chen YM, Chen LH, Li MP, Li HF, Higuchi A, Kumar SS, Ling QD, Alarfaj AA, Munusamy MA, Chang Y, Benelli G, Murugan K, Umezawa A.  Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.  Sci Rep  2017  7:45146  PubMed ID: 28332572   DOI: 10.1038/srep45146

10373  Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.  Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.  Stem Cell Res  2017  23:13-19  PubMed ID: 28666145   DOI: 10.1016/j.scr.2017.06.011

4551  Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K.  Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells.  Nat Protoc  2017  12(4):683-696  PubMed ID: 28253236   DOI: 10.1038/nprot.2017.007

7080  Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835   DOI: 10.1038/nature17000

9686  Konagaya S, Iwata H.  Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study.  Biochim. Biophys. Acta  2016  1860:2008-16  PubMed ID: 27180174   DOI: 10.1016/j.bbagen.2016.05.012

7112  Higuchi A, Kao SH, Ling QD, Chen YM, Li HF, Alarfaj AA, Munusamy MA, Murugan K, Chang SC, Lee HC, Hsu ST, Kumar SS, Umezawa A.  Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.  Sci Rep  2015  5:18136  PubMed ID: 26656754   DOI: 10.1038/srep18136

7213  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514   DOI: 10.1016/j.stemcr.2015.08.008

5790  Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.  Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.  Sci Rep  2015  5:16647  PubMed ID: 26573336   DOI: 10.1038/srep16647

5792  Nagaoka M, Kobayashi M, Kawai C, Mallanna SK, Duncan SA.  Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.  PLoS One  2015  10(8):e0136350  PubMed ID: 26308339   DOI: 10.1371/journal.pone.0136350

15246  Higuchi A, Wang CT, Ling QD, Lee HH, Kumar SS, Chang Y, Alarfaj AA, Munusamy MA, Hsu ST, Wu GJ, Umezawa A.  A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues  Sci Rep  2015  5:10217  PubMed ID: 25970301   DOI: 10.1038/srep10217

5787  Kamao H, Mandai M, Wakamiya S, Ishida J, Goto K, Ono T, Suda T, Takahashi M, Kiryu J.  Objective evaluation of the degree of pigmentation in human induced pluripotent stem cell-derived RPE.  Invest Ophthalmol Vis Sci  2014  55(12):8309-18.  PubMed ID: 25389202   DOI: 10.1167/iovs.14-14694