Cell No. : Cell Name HPS0077 : 454E2  update : 2020/12/17
Comment			Human iPS cell line. Application consideration. Order Form (C-0042, C-0034). Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions			1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
	Basic information	Depositor	http://www.cira.kyoto-u.ac.jp/
		Originator	Yamanaka, Shinya
		Year of deposit	2011
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	16 years
		Tissue	dental pulp
		Classification	iPS
		Recombinant	recombinant
		Exogene	pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
		Lifespan	infinite
		Morphology	ES-like
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		Primate ES Cell Medium + 4ng/ml human basic FGF
		Antibiotics		Free
		Passage method		0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage ratio		1 : 3-5 split
		SC frequency		Subculture : once/3-5 days, Medium Renewal : every day
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		SNL 76/7
		Feeder treatment		X-rays 5000R (or MMC)
		Feeder seed cells		1.5-2.5 x 10^(6) cells/100 mm dish
		Freeze medium		DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
		Freezing method		Vitrification
		Mycoplasma		(-)
		STR(human)		OK
		Chromosome mode		46XX(20)
Images			deposit info	lot info
Relational File			deposit info	lot info
				SDS_DAP(MEM)_Japanese.pdf
Reference information		Reference	1
		User's Publication	26

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Reference

3676  Okita K1, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.  A more efficient method to generate integration-free human iPS cells.  Nat Methods  2011  8(5):409-12  PubMed ID: 21460823

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User's Publication

6879  Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H.  Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells.  Cell Rep  2020  30(1):18-24  PubMed ID: 31914385

6969  Aoyama J, Homma K, Tanabe N, Usui S, Miyagi Y, Matsuura K, Kaneda M, Nitta T.  Spatiotemporal imaging documented the maturation of the cardiomyocytes from human induced pluripotent stem cells.  J Thorac Cardiovasc Surg  2020  159(6):2260-2271.e7  PubMed ID: 31409490

12042  Ninomiya H, Intoh A, Ishimine H, Onuma Y, Ito Y, Michiue T, Tazaki A, Kato M.  Application of a human mesoderm tissue elongation system in vitro derived from human induced pluripotent stem cells to risk assessment for teratogenic chemicals.  Chemosphere  2020    PubMed ID: 32092576

11745  Sung TC, Yang JS, Yeh CC, Liu YC, Jiang YP, Lu MW, Ling QD, Kumar SS, Chang Y, Umezawa A, Chen H, Higuchi A.  The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells.  Biomaterials  2019    PubMed ID: 31419657

11798  Sugawara Y, Hamada K, Yamada Y, Kumai J, Kanagawa M, Kobayashi K, Toda T, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Kikkawa Y.  Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment.  Sci Rep  2019    PubMed ID: 31506597

6894  Chen LH , Sung TC , Lee HH , Higuchi A , Su HC , Lin KJ , Huang YR , Ling QD , Kumar SS , Alarfaj AA , Munusamy MA , Nasu M , Chen DC , Hsu ST , Chang Y , Lee KF , Wang HC , Umezawa A .  Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels.  Biomater Sci  2019  7(10):4345-4362  PubMed ID: 31411209

12369  Kamao H, Miki A, Kiryu J.  Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activat  J Ophthalmol  2019  2019:7189241  PubMed ID: 31016039

10713  Kosmidou C, Efstathiou NE, Hoang MV, Notomi S, Konstantinou EK, Hirano M, Takahashi K, Maidana DE, Tsoka P, Young L, Gragoudas ES, Olsen TW, Morizane Y, Miller JW, Vavvas DG.  Issues with the Specificity of Immunological Reagents for NLRP3: Implications for Age-related Macular Degeneration.  Sci Rep  2018  8:461  PubMed ID: 29323137

11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143

4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2018    PubMed ID: 30114722

5773  Kim MH, Matsubara Y, Fujinaga Y, Kino-Oka M.  A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin.  Biotechnol J  2018  13(2)  PubMed ID: 29027750

7022  Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.  CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.  Nat. Methods  2017  14:963-966  PubMed ID: 28892089

7026  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162

10064  Lin HR, Heish CW, Liu CH, Muduli S, Li HF, Higuchi A, Kumar SS, Alarfaj AA, Munusamy MA, Hsu ST, Chen DC, Benelli G, Murugan K, Cheng NC, Wang HC, Wu GJ.  Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods.  Sci Rep  2017  7:40069  PubMed ID: 28071738

10071  Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582

10093  Homma K, Usui S, Kaneda M.  Knock-in strategy at 3'-end of Crx gene by CRISPR/Cas9 system shows the gene expression profiles during human photoreceptor differentiation.  Genes Cells  2017  22:250-264  PubMed ID: 28124402

10216  Chen YM, Chen LH, Li MP, Li HF, Higuchi A, Kumar SS, Ling QD, Alarfaj AA, Munusamy MA, Chang Y, Benelli G, Murugan K, Umezawa A.  Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.  Sci Rep  2017  7:45146  PubMed ID: 28332572

10373  Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.  Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.  Stem Cell Res  2017  23:13-19  PubMed ID: 28666145

4551  Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K.  Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells.  Nat Protoc  2017  12(4):683-696  PubMed ID: 28253236

7080  Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835

9686  Konagaya S, Iwata H.  Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study.  Biochim. Biophys. Acta  2016  1860:2008-16  PubMed ID: 27180174

7112  Higuchi A, Kao SH, Ling QD, Chen YM, Li HF, Alarfaj AA, Munusamy MA, Murugan K, Chang SC, Lee HC, Hsu ST, Kumar SS, Umezawa A.  Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.  Sci Rep  2015  5:18136  PubMed ID: 26656754

7213  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514

5790  Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.  Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.  Sci Rep  2015  5:16647  PubMed ID: 26573336

5792  Nagaoka M, Kobayashi M, Kawai C, Mallanna SK, Duncan SA.  Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.  PLoS One  2015  10(8):e0136350  PubMed ID: 26308339

5787  Kamao H, Mandai M, Wakamiya S, Ishida J, Goto K, Ono T, Suda T, Takahashi M, Kiryu J.  Objective evaluation of the degree of pigmentation in human induced pluripotent stem cell-derived RPE.  Invest Ophthalmol Vis Sci  2014  55(12):8309-18.  PubMed ID: 25389202