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Cell No. : Cell Name
HPS0076 : 409B2  update : 2020/11/11
CommentHuman iPS cell line. HPS0002, HPS0063, HPS0076, HPS0296, HPS0326, HPS3668, HPS4290, HPS4291, HPS4292 are derived from the same person. Order Form (C-0042, C-0007 or C-0007p).
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach TERMS and CONDITIONS (non-for-profit) or TERMS and CONDITIONS (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 36 years
Tissue skin
Classification iPS
Recombinant recombinant
Exogene pCXLE, human Oct3/4, human Sox2, human Klf4, human L-Myc, human Lin28, p53 shRNA
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Chromosome mode 46XX(20)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 2
User's Publication 32


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Reference
4005  Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N, Inoue H.  iPSC-Based Compound Screening and In Vitro Trials Identify a Synergistic Anti-amyloid β Combination for Alzheimer's Disease.  Cell Rep  2017  21:2304-2312  PubMed ID: 29166618
3676  Okita K1, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.  A more efficient method to generate integration-free human iPS cells.  Nat Methods  2011  8(5):409-12  PubMed ID: 21460823

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User's Publication
6879  Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H.  Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells.  Cell Rep  2020  30(1):18-24  PubMed ID: 31914385
10143  Tomoyo Sawada, Thomas E Chater, Yohei Sasagawa, Mika Yoshimura, Noriko Fujimori-Tonou, Kaori Tanaka, Kynon J M Benjamin, Apuã C M Paquola, Jennifer A Erwin, Yukiko Goda, Itoshi Nikaido, Tadafumi Kato  Developmental excitation-inhibition imbalance underlying psychoses revealed by single-cell analyses of discordant twins-derived cerebral organoids  Mol Psychiatry  2020    PubMed ID: 32764691
11989  Dayem AA, Won J, Goo HG, Yang GM, Seo DS, Jeon BM, Choi HY, Park SE, Lim KM, Jang SH, Lee SB, Choi SB, Kim K, Kang GH, Yeon GB, Kim DS, Cho SG.  The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells.  Stem Cell Res  2020    PubMed ID: 31981882
11304  Ueda M, Hashimoto Y, Honda Y, Baba S, Morita S.  Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions.  Med Mol Morphol  2019    PubMed ID: 30446810
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818
5346  Kirihara T, Luo Z, Chow SYA, Misawa R, Kawada J, Shibata S, Khoyratee F, Vollette CA, Volz V, Levi T, Fujii T, Ikeuchi Y.  A Human Induced Pluripotent Stem Cell-Derived Tissue Model of a Cerebral Tract Connecting Two Cortical Regions.  iScience  2019  14:301-311  PubMed ID: 31006610
11576  Komatsu Y, Takeuchi D, Tokunaga T, Sakurai H, Makino A, Honda T, Ikeda Y, Tomonaga K.  RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs.  Mol Ther Methods Clin Dev  2019  14:47-55  PubMed ID: 31309127
11589  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Biochem. Biophys. Res. Commun.  2019  515(4):672-678  PubMed ID: 31178142
11744  Riesenberg S, Chintalapati M, Macak D, Kanis P, Maricic T, Pääbo S.  Simultaneous precise editing of multiple genes in human cells.  Nucleic Acids Res.  2019    PubMed ID: 31392986
11860  Kanton S, Boyle MJ, He Z, Santel M, Weigert A, Sanchís-Calleja F, Guijarro P, Sidow L, Fleck JS, Han D, Qian Z, Heide M, Huttner WB, Khaitovich P, Pääbo S, Treutlein B, Camp JG.  Organoid single-cell genomic atlas uncovers human-specific features of brain development.  Nature  2019    PubMed ID: 31619793
10578  Yuki Maeda, Keiichi Motoyama, Taishi Higashi, Risako Onodera, Toru Takeo, Naomi Nakagata, Yuki Kurauchi, Hiroshi Katsuki, Yoichi Ishitsuka, Yuki Kondo, Tetsumi Irie, Takumi Era, Hidetoshi Arima  Lowering effect of dimethyl-α-cyclodextrin on GM1-ganglioside accumulation in GM1-gangliosidosis model cells and in brain of β-galactosidase-knockout mice  Journal of Inclusion Phenomena and Macrocyclic Chemistry  2019  93:53–66 
7001  Akiyama T, Sato S, Chikazawa-Nohtomi N, Soma A, Kimura H, Wakabayashi S, Ko SBH, Ko MSH.  Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.  Sci Rep  2018  8:1189  PubMed ID: 29352121
10463  Sato-Nishiuchi R, Li S, Ebisu F, Sekiguchi K.  Recombinant laminin fragments endowed with collagen-binding activity: A tool for conferring laminin-like cell-adhesive activity to collagen matrices.  Matrix Biol.  2018  65:75-90  PubMed ID: 28801205
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143
4124  Ikeda K, Kawasaki Y, Matsuda H, Kishida S, Iwasaki A, Ohata S, Urashima Y, Hirotani Y.  Approach for differentiating trophoblast cell lineage from human induced pluripotent stem cells with retinoic acid in the absence of bone morphogenetic protein 4.  Placenta  2018    PubMed ID: 30415744
5348  Yoshinori Hasegawa, Masashi Ikeno, Nobutaka Suzuki, Manabu Nakayama, Osamu Ohara  Improving the efficiency of gene insertion in a human artificial chromosome vector and its transfer in human-induced pluripotent stem cells  Biology Methods and Protocols  2018  1–10 
7009  Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe SI, Nishina S, Azuma N.  Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.  Sci Rep  2017  7:16757  PubMed ID: 29196712
7015  Kawada J, Kaneda S, Kirihara T, Maroof A, Levi T, Eggan K, Fujii T, Ikeuchi Y.  Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons.  Stem Cell Reports  2017  9:1441-1449  PubMed ID: 29107592
7026  Koui Y, Kido T, Ito T, Oyama H, Chen SW, Katou Y, Shirahige K, Miyajima A.  An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells.  Stem Cell Reports  2017  9:490-498  PubMed ID: 28757162
7031  Ikeda K, Nagata S, Okitsu T, Takeuchi S.  Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.  Sci Rep  2017  7:2850  PubMed ID: 28588295
9918  Shimizu Y, Satou M, Hayashi K, Nakamura Y, Fujimaki M, Horibata Y, Ando H, Watanabe T, Shiobara T, Chibana K, Takemasa A, Sugimoto H, Anzai N, Ishii Y.  Matrix-assisted laser desorption/ionization imaging mass spectrometry reveals changes of phospholipid distribution in induced pluripotent stem cell colony differentiation.  Anal Bioanal Chem  2017  409:1007-1016  PubMed ID: 27815610
10345  Zuiki M, Chiyonobu T, Yoshida M, Maeda H, Yamashita S, Kidowaki S, Hasegawa T, Gotoh H, Nomura T, Ono K, Hosoi H, Morimoto M.  Luteolin attenuates interleukin-6-mediated astrogliosis in human iPSC-derived neural aggregates: A candidate preventive substance for maternal immune activation-induced abnormalities.  Neurosci. Lett.  2017  653:296-301  PubMed ID: 28595950
10409  Bamba Y, Kanemura Y, Okano H, Yamasaki M.  Visualization of migration of human cortical neurons generated from induced pluripotent stem cells.  J. Neurosci. Methods  2017  289:57-63  PubMed ID: 28694214
5774  Miyagoe-Suzuki Y, Nishiyama T, Nakamura M, Narita A, Takemura F, Masuda S, Minami N, Murayama K, Komaki H, Goto YI, Takeda S.  Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status.  Stem Cells Int  2017  2017:7906843  PubMed ID: 28491099
6234  Nakamura Y, Shimizu Y, Horibata Y, Tei R, Koike R, Masawa M, Watanabe T, Shiobara T, Arai R, Chibana K, Takemasa A, Sugimoto H, Ishii Y.  Changes of plasmalogen phospholipid levels during differentiation of induced pluripotent stem cells 409B2 to endothelial phenotype cells.  Sci Rep  2017  7(1):9377  PubMed ID: 28839272
7053  Maeda T, Nagano S, Ichise H, Kataoka K, Yamada D, Ogawa S, Koseki H, Kitawaki T, Kadowaki N, Takaori-Kondo A, Masuda K, Kawamoto H.  Regeneration of CD8αβ T Cells from T-cell-Derived iPSC Imparts Potent Tumor Antigen-Specific Cytotoxicity.  Cancer Res.  2016  76:6839-6850  PubMed ID: 27872100
9858  Mora-Bermúdez F, Badsha F, Kanton S, Camp JG, Vernot B, Köhler K, Voigt B, Okita K, Maricic T, He Z, Lachmann R, Pääbo S, Treutlein B, Huttner WB.  Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development.  Elife  2016  5:  PubMed ID: 27669147
7213  Kido T, Koui Y, Suzuki K, Kobayashi A, Miura Y, Chern EY, Tanaka M, Miyajima A.  CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells.  Stem Cell Reports  2015  5:508-15  PubMed ID: 26365514
5451  Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N.  Generation of retinal ganglion cells with functional axons from human induced pluripotent stem cells.  Sci Rep  2015  5:8344  PubMed ID: 25666360
6231  Baden MY, Fukui K, Hosokawa Y, Iwahashi H, Imagawa A, Shimomura I.  Examination of a Viral Infection Mimetic Model in Human iPS Cell-Derived Insulin-Producing Cells and the Anti-Apoptotic Effect of GLP-1 Analogue.  PLoS One  2015  10(12):e0144606  PubMed ID: 26659307
5741  Tano K, Yasuda S, Kuroda T, Saito H, Umezawa A, Sato Y.  A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system.  PLoS One  2014  9(10):e110496  PubMed ID: 25347300
9954  Morgan BJ, Crabtree D, Skatrud JB.  Chemoreflex sensitization augments sympathetic vasomotor outflow in awake humans.  Adv. Exp. Med. Biol.  1994  360:269-71  PubMed ID: 7872100



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