Cell No. : Cell Name
HPS0045 : HiPS-RIKEN-5A
update : 2023/06/07
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| Comment | Disease specific iPS cell line derived from a patient : Wilson's disease (RCB0395 NCU-F8). The analysis performed by RIKEN BRC indicated mutations in ATP7B gene; 2333G>T (p.R778L) in one allele and 2495A>G (p.K832R) and c.2855G>A (p.R952K) in another allele. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | 1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Contact Us : RIKEN Cell Bank |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nakamura, Yukio
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| Originator |
Shimizu, Natsumi
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| Year of deposit |
2010
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Race |
Japanese
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| Gender |
Female
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| Tissue |
skin
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| Disease name |
Wilson's disease
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_B519
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/3-5 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1.0-1.5 x 10 6 cells/100 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| Chromosome mode |
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46XX(20)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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| Reference |
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16703
Song D, Takahashi G, Zheng YW, Matsuo-Takasaki M, Li J, Takami M, An Y, Hemmi Y, Miharada N, Fujioka T, Noguchi M, Nakajima T, Saito MK, Nakamura Y, Oda T, Miyaoka Y, Hayashi Y.
Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes
Hum Mol Genet
2022
Apr 7;ddac080
PubMed ID: 35388883
DOI: 10.1093/hmg/ddac080
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