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Cell No. : Cell Name
HPS0045 : HiPS-RIKEN-5A  update : 2023/06/07
CommentDisease specific iPS cell line derived from a patient : Wilson's disease (RCB0395 NCU-F8). The analysis performed by RIKEN BRC indicated mutations in ATP7B gene; 2333G>T (p.R778L) in one allele and 2495A>G (p.K832R) and c.2855G>A (p.R952K) in another allele. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Contact Us : RIKEN Cell Bank
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakamura, Yukio
Originator Shimizu, Natsumi
Year of deposit 2010
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue skin
Disease name Wilson's disease
Classification iPS
Recombinant recombinant
Exogene pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B519
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
Chromosome mode 46XX(20)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 0


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Reference
16703  Song D, Takahashi G, Zheng YW, Matsuo-Takasaki M, Li J, Takami M, An Y, Hemmi Y, Miharada N, Fujioka T, Noguchi M, Nakajima T, Saito MK, Nakamura Y, Oda T, Miyaoka Y, Hayashi Y.  Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes  Hum Mol Genet  2022  Apr 7;ddac080  PubMed ID: 35388883   DOI: 10.1093/hmg/ddac080

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