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Cell No. : Cell Name
HPS0014 : HiPS-RIKEN-2F  update : 2021/08/19
CommentHuman iPS cell line. Parent cell line of RCB0197 HUC-Fm.
Comment from the depositor
Terms and conditionsIn relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells.
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakamura, Yukio
Originator Fujioka, Tsuyoshi
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue umbilical cord
Classification iPS
Recombinant recombinant
Exogene pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_1Q63
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 2
User's Publication 3


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Reference
9005  Noriko Itaba 1, Peninah M Wairagu, Natsumi Aramaki, Toshihiro Yasui, Yoshiaki Matsumi, Yohei Kono, Ai N H Phan, Makoto Otsu, Takahiro Kunisada, Yukio Nakamura, Hideyuki Okano, Yangsik Jeong, Goshi Shiota  Nuclear Receptor Gene Alteration in Human Induced Pluripotent Stem Cells With Hepatic Differentiation Propensity  Hepatol Res  2014  44 (14), E408-19  PubMed ID: 24636009   DOI: 10.1111/hepr.12329
9174  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836   DOI: 10.1111/j.1749-0774.2010.00091.x

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User's Publication
4054  Miyoshi M, Kakinuma S, Kamiya A, Tsunoda T, Tsuchiya J, Sato A, Kaneko S, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Asahina Y, Watanabe M.  LIM homeobox 2 promotes interaction between human iPS-derived hepatic progenitors and iPS-derived hepatic stellate-like cells.  Sci Rep  2019  9:2072  PubMed ID: 30765795   DOI: 10.1038/s41598-018-37430-9
9010  Akihide Kamiya, Kazuya Anzai, Kota Tsuruya, Hiromi Chikada  Culture System of Bile Duct-Like Cystic Structures Derived From Human-Inducible Pluripotent Stem Cells  Methods Mol Biol   2019  1905, 143-153   PubMed ID: 30536097   DOI: 10.1007/978-1-4939-8961-4_13
5448  Kaneko S, Kakinuma S, Asahina Y, Kamiya A, Miyoshi M, Tsunoda T, Nitta S, Asano Y, Nagata H, Otani S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Nakauchi H, Nishitsuji H, Ujino S, Shimotohno K, Iwamoto M, Watashi K, Wakita T, Watanabe M.  Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus.  Sci Rep  2016  6:29358  PubMed ID: 27386799   DOI: 10.1038/srep29358



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