細胞番号 : 細胞名
HPS0009 : HiPS-RIKEN-2A
update : 2023/06/07
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| 細胞特性(Comment:英) | Human iPS cell line. Parent cell line of RCB0197 HUC-Fm. Cryopreserved by vitrification method. |
| 細胞特性(日) | ヒト人工多能性幹（iPS）細胞株。臍帯由来線維芽細胞 (RCB0197 HUC-Fm) にレトロウイルスベクターにより4因子（Oct3/4, Sox2, Klf4, c-Myc）を導入して樹立。ガラス化法（Vitrification法）保存。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. |
| 使用条件(日) | 非営利機関以外の利用者は事前にiPSアカデミアジャパン株式会社 から確認書を得ること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0041.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Nakamura, Yukio
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| 樹立者 |
Fujioka, Tsuyoshi
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| 寄託日 |
2008
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 人種 |
Japanese
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| 性別 |
Male
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| 採取組織 |
umbilical cord
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| 細胞分類 |
iPS
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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| Cellosaurus(Expasy) |
CVCL_B512
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| 培養容器のコーティング |
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0.1% gelatin coated dish
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継代密度 |
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1 : 2-4 split
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| 継代・培地交換頻度 |
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Subculture : once/3-5 days, Medium Renewal : every day
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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3
%
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| フィーダー細胞 |
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MEF
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| フィーダー細胞の処理方法 |
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X-rays 5000R (or MMC)
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| フィーダー細胞の播種数 |
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1.0-1.5 x 10 6 cells/100 mm dish
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| 凍結培地 |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| 凍結方法 |
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Vitrification
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| マイコプラズマ/アコレプラズマ |
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(-)
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| 染色体検査 |
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46XY(20)
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| その他の細胞情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
3件
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| Reference(日) |
0件
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| 利用者成果(英) |
12件
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| 利用者成果(日) |
0件
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| Reference(英) |
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9005
Noriko Itaba 1, Peninah M Wairagu, Natsumi Aramaki, Toshihiro Yasui, Yoshiaki Matsumi, Yohei Kono, Ai N H Phan, Makoto Otsu, Takahiro Kunisada, Yukio Nakamura, Hideyuki Okano, Yangsik Jeong, Goshi Shiota
Nuclear Receptor Gene Alteration in Human Induced Pluripotent Stem Cells With Hepatic Differentiation Propensity
Hepatol Res
2014
44 (14), E408-19
PubMed ID: 24636009
DOI: 10.1111/hepr.12329
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9002
Naoki Fujitani , Jun-ichi Furukawa, Kayo Araki, Tsuyoshi Fujioka, Yasuhiro Takegawa, Jinhua Piao, Taiki Nishioka, Tomohiro Tamura, Toshio Nikaido, Makoto Ito, Yukio Nakamura, Yasuro Shinohara
Total Cellular Glycomics Allows Characterizing Cells and Streamlining the Discovery Process for Cellular Biomarkers
Proc Natl Acad Sci U S A
2013
110 (6), 2105-10
PubMed ID: 23345451
DOI: 10.1073/pnas.1214233110
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9174
Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.
Establishment of induced pluripotent stem cells from human neonatal tissues.
Hum Cell
2010
23(3):113-8
PubMed ID: 20973836
DOI: 10.1111/j.1749-0774.2010.00091.x
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| 利用者成果(英) |
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21546
Kuroda T, Yasuda S, Matsuyama S, Miura T, Sawada R, Matsuyama A, Yamamoto Y, Morioka MS, Kawaji H, Kasukawa T, Itoh M, Akutsu H, Kawai J, Sato Y.
ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons.
Sci Rep
2024
14(1):690
PubMed ID: 38184695
DOI: 10.1038/s41598-023-51082-4
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17600
Kudo KI, Tsuyama N, Nagata K, Imaoka T, Iizuka D, Sugai-Takahashi M, Muramatsu M, Sakai A.
ΔNp63α transcriptionally represses p53 target genes involved in the radiation-induced DNA damage response : ΔNp63α may cause genomic instability in epithelial stem cells
Radiat Oncol
2022
17(1):183
PubMed ID: 36380314
DOI: 10.1186/s13014-022-02139-7
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20724
Yasui R, Matsui A, Sekine K, Okamoto S, Taniguchi H.
Highly Sensitive Detection of Human Pluripotent Stem Cells by Loop-Mediated Isothermal Amplification.
Stem Cell Rev Rep
2022
PubMed ID: 35661077
DOI: 10.1007/s12015-022-10402-3
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18126
Panina Y, Yamane J, Kobayashi K, Sone H, Fujibuchi W.
Human ES and iPS cells display less drug resistance than differentiated cells, and naïve-state induction further decreases drug resistance
J Toxicol Sci
2021
46(3):131-142
PubMed ID: 33642519
DOI: 10.2131/jts.46.131
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13411
Yasui R, Sekine K, Yamaguchi K, Furukawa Y, Taniguchi H.
Robust parameter design of human induced pluripotent stem cell differentiation protocols defines lineage-specific induction of anterior-posterior gut tube endodermal cells.
Stem Cells
2021
PubMed ID: 33400835
DOI: 10.1002/stem.3326
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4117
Csobonyeiova M, Polak S, Danisovic L.
Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.
Exp. Clin. Endocrinol. Diabetes
2020
PubMed ID: 30114722
DOI: 10.1055/a-0661-5873
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13769
Yulia Panina, Junko Yamane, Kenta Kobayashi, Hideko Sone, Wataru Fujibuchi
Cell Pluripotency and Its Effect on Sensitivity to Chemical Exposure
bioRxiv
2020
DOI: 10.1101/2020.08.17.253492
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4572
Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.
SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.
Nat Commun
2019
10(1):2175
PubMed ID: 31092818
DOI: 10.1038/s41467-019-09511-4
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11234
Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.
Tumorigenicity-associated characteristics of human iPS cell lines.
PLoS ONE
2018
PubMed ID: 30286143
DOI: 10.1371/journal.pone.0205022
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9686
Konagaya S, Iwata H.
Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study.
Biochim. Biophys. Acta
2016
1860:2008-16
PubMed ID: 27180174
DOI: 10.1016/j.bbagen.2016.05.012
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7562
Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato
Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.
Regenerative Therapy
2015
2:17-23
PubMed ID: 31245455
DOI: 10.1016/j.reth.2015.08.001
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5790
Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.
Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.
Sci Rep
2015
5:16647
PubMed ID: 26573336
DOI: 10.1038/srep16647
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細胞材料の再培養方法 (
HPS以外・
HPS )
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細胞材料開発室サイト
SDS_DAP(MEM)_Japanese.pdf