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Cell No. : Cell Name
HPS0009 : HiPS-RIKEN-2A  update : 2023/06/07
CommentHuman iPS cell line. Parent cell line of RCB0197 HUC-Fm. Cryopreserved by vitrification method.
Comment from the depositor
Terms and conditionsIn relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells.
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakamura, Yukio
Originator Fujioka, Tsuyoshi
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue umbilical cord
Classification iPS
Recombinant recombinant
Exogene pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B512
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.0-1.5 x 10 6 cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma/Acholeplasma (-)
Chromosome mode 46XY(20)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 3
User's Publication 12


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Reference
9005  Noriko Itaba 1, Peninah M Wairagu, Natsumi Aramaki, Toshihiro Yasui, Yoshiaki Matsumi, Yohei Kono, Ai N H Phan, Makoto Otsu, Takahiro Kunisada, Yukio Nakamura, Hideyuki Okano, Yangsik Jeong, Goshi Shiota  Nuclear Receptor Gene Alteration in Human Induced Pluripotent Stem Cells With Hepatic Differentiation Propensity  Hepatol Res  2014  44 (14), E408-19  PubMed ID: 24636009   DOI: 10.1111/hepr.12329
9002  Naoki Fujitani , Jun-ichi Furukawa, Kayo Araki, Tsuyoshi Fujioka, Yasuhiro Takegawa, Jinhua Piao, Taiki Nishioka, Tomohiro Tamura, Toshio Nikaido, Makoto Ito, Yukio Nakamura, Yasuro Shinohara  Total Cellular Glycomics Allows Characterizing Cells and Streamlining the Discovery Process for Cellular Biomarkers  Proc Natl Acad Sci U S A   2013  110 (6), 2105-10  PubMed ID: 23345451   DOI: 10.1073/pnas.1214233110
9174  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836   DOI: 10.1111/j.1749-0774.2010.00091.x

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User's Publication
21546  Kuroda T, Yasuda S, Matsuyama S, Miura T, Sawada R, Matsuyama A, Yamamoto Y, Morioka MS, Kawaji H, Kasukawa T, Itoh M, Akutsu H, Kawai J, Sato Y.  ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons.  Sci Rep  2024  14(1):690  PubMed ID: 38184695   DOI: 10.1038/s41598-023-51082-4
17600  Kudo KI, Tsuyama N, Nagata K, Imaoka T, Iizuka D, Sugai-Takahashi M, Muramatsu M, Sakai A.  ΔNp63α transcriptionally represses p53 target genes involved in the radiation-induced DNA damage response : ΔNp63α may cause genomic instability in epithelial stem cells  Radiat Oncol  2022  17(1):183  PubMed ID: 36380314   DOI: 10.1186/s13014-022-02139-7
20724  Yasui R, Matsui A, Sekine K, Okamoto S, Taniguchi H.  Highly Sensitive Detection of Human Pluripotent Stem Cells by Loop-Mediated Isothermal Amplification.  Stem Cell Rev Rep  2022    PubMed ID: 35661077   DOI: 10.1007/s12015-022-10402-3
18126  Panina Y, Yamane J, Kobayashi K, Sone H, Fujibuchi W.  Human ES and iPS cells display less drug resistance than differentiated cells, and naïve-state induction further decreases drug resistance  J Toxicol Sci  2021  46(3):131-142  PubMed ID: 33642519   DOI: 10.2131/jts.46.131
13411  Yasui R, Sekine K, Yamaguchi K, Furukawa Y, Taniguchi H.  Robust parameter design of human induced pluripotent stem cell differentiation protocols defines lineage-specific induction of anterior-posterior gut tube endodermal cells.  Stem Cells  2021    PubMed ID: 33400835   DOI: 10.1002/stem.3326
4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2020    PubMed ID: 30114722   DOI: 10.1055/a-0661-5873
13769  Yulia Panina, Junko Yamane, Kenta Kobayashi, Hideko Sone, Wataru Fujibuchi  Cell Pluripotency and Its Effect on Sensitivity to Chemical Exposure  bioRxiv  2020      DOI: 10.1101/2020.08.17.253492
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818   DOI: 10.1038/s41467-019-09511-4
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143   DOI: 10.1371/journal.pone.0205022
9686  Konagaya S, Iwata H.  Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study.  Biochim. Biophys. Acta  2016  1860:2008-16  PubMed ID: 27180174   DOI: 10.1016/j.bbagen.2016.05.012
7562  Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23  PubMed ID: 31245455   DOI: 10.1016/j.reth.2015.08.001
5790  Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.  Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.  Sci Rep  2015  5:16647  PubMed ID: 26573336   DOI: 10.1038/srep16647


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