Cell No. : Cell Name
HPS0003 : HiPS-RIKEN-1A
update : 2023/07/27
|
| Comment | Human iPS cell line. Parent cell line of RCB0436 HUC-F2. Cryopreserved by vitrification method. |
| Comment from the depositor | |
| Terms and conditions | In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. |
| Remarks | |
| Order Form |
Order Form(C-0042.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Nakamura, Yukio
|
| Originator |
Fujioka, Tsuyoshi
|
| Year of deposit |
2008
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Tissue |
umbilical cord
|
| Classification |
iPS
|
| Recombinant |
recombinant
|
| Exogene |
pMXs-Oct3/4, -Sox2, -Klf4, -c-Myc
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_B511
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage ratio |
|
1 : 2-4 split
|
| SC frequency |
|
Subculture : once/3-5 days, Medium Renewal : every day
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
3
%
|
| Feeder cells |
|
MEF
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
|
| Feeder seed cells |
|
1.0-1.5 x 10 6 cells/100 mm dish
|
| Freeze medium |
|
DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
|
| Freezing method |
|
Vitrification
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Chromosome mode |
|
46XX(18), 47XX+X(2)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Relational File |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
2
|
| User's Publication |
6
|
| Reference |
|
9002
Naoki Fujitani , Jun-ichi Furukawa, Kayo Araki, Tsuyoshi Fujioka, Yasuhiro Takegawa, Jinhua Piao, Taiki Nishioka, Tomohiro Tamura, Toshio Nikaido, Makoto Ito, Yukio Nakamura, Yasuro Shinohara
Total Cellular Glycomics Allows Characterizing Cells and Streamlining the Discovery Process for Cellular Biomarkers
Proc Natl Acad Sci U S A
2013
110 (6), 2105-10
PubMed ID: 23345451
DOI: 10.1073/pnas.1214233110
|
|
9174
Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.
Establishment of induced pluripotent stem cells from human neonatal tissues.
Hum Cell
2010
23(3):113-8
PubMed ID: 20973836
DOI: 10.1111/j.1749-0774.2010.00091.x
|
| User's Publication |
|
21544
Ueki M, Suzuki T, Kato Y.
Large-scale cultivation of human iPS cells in bioreactor with reciprocal mixing.
J Biosci Bioeng
2024
PubMed ID: 38185598
DOI: 10.1016/j.jbiosc.2023.12.008
|
|
21546
Kuroda T, Yasuda S, Matsuyama S, Miura T, Sawada R, Matsuyama A, Yamamoto Y, Morioka MS, Kawaji H, Kasukawa T, Itoh M, Akutsu H, Kawai J, Sato Y.
ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons.
Sci Rep
2024
14(1):690
PubMed ID: 38184695
DOI: 10.1038/s41598-023-51082-4
|
|
4572
Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.
SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.
Nat Commun
2019
10(1):2175
PubMed ID: 31092818
DOI: 10.1038/s41467-019-09511-4
|
|
11234
Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.
Tumorigenicity-associated characteristics of human iPS cell lines.
PLoS ONE
2018
PubMed ID: 30286143
DOI: 10.1371/journal.pone.0205022
|
|
7562
Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato
Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.
Regenerative Therapy
2015
2:17-23
PubMed ID: 31245455
DOI: 10.1016/j.reth.2015.08.001
|
|
16884
Maeda T, Lee MJ, Palczewska G, Marsili S, Tesar PJ, Palczewski K, Takahashi M, Maeda A.
Retinal pigmented epithelial cells obtained from human induced pluripotent stem cells possess functional visual cycle enzymes in vitro and in viv
J Biol Chem
2013
288(48):34484-9
PubMed ID: 24129572
DOI: 10.1074/jbc.M113.518571
|
Back
Top
CELL BANK Website
SDS_DAP(MEM)_Japanese.pdf