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Cell No. : Cell Name
HPS0002 : 253G1  update : 2022/11/29
CommentHuman iPS cell line. HPS0002, HPS0063, HPS0076, HPS0296, HPS0326, HPS3668, HPS4290, HPS4291, HPS4292 were derived from the same person.
Comment from the depositor
Terms and conditions1) In relation to the for-profit organizations, please contact iPS Academia Japan, Inc., prior to the utilization of iPS cells. 2) Attach Appendix (non-for-profit) or Appendix (for-profit) to MTA (C-0007 or C-0007p) .
Remarks
Order Form Order Form(C-0042.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor https://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Female
Age at sampling 36 years
Tissue skin
Classification iPS
Recombinant recombinant
Exogene pMXs, Oct3/4, Sox2, Klf4, pLenti6/UbC/V5-DEST, mouse Slc7a1
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B518
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Primate ES Cell Medium + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-5 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
Chromosome mode 46,XX[20] at passage No. 23
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 123


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Reference
3673  Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S.  Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.  Nat Biotechnol  2008  26(1):101-6  PubMed ID: 18059259   DOI: 10.1038/nbt1374

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User's Publication
16925  Nakazato T, Kawamura T, Uemura T, Liu L, Li J, Sasai M, Harada A, Ito E, Iseoka H, Toda K, Sawa Y, Miyagawa S.  Engineered three-dimensional cardiac tissues maturing in a rotating wall vessel bioreactor remodel diseased hearts in rats with myocardial infarction  Stem Cell Reports  2022  17(5):1170-1182  PubMed ID: 35427484   DOI: 10.1016/j.stemcr.2022.03.012
20948  Shirasago Y, Fukazawa H, Nagase S, Shimizu Y, Mizukami T, Wakita T, Suzuki T, Tani H, Kondoh M, Kuroda T, Yasuda S, Sato Y, Hanada K, Fukasawa M.  A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection.  Sci Rep  2022  12(1):20243  PubMed ID: 36424447   DOI: 10.1038/s41598-022-23824-3
12690  Yoshida K, Uehara O, Kurashige Y, Paudel D, Onishi A, Neopane P, Hiraki D, Morikawa T, Harada F, Takai R, Sato J, Saitoh M, Abiko Y.  Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents  Sci Rep  2021  11(1):1852  PubMed ID: 33473142   DOI: 10.1038/s41598-020-79426-4
13203  Uto S, Hikita A, Sakamoto T, Mori D, Yano F, Ohba S, Saito T, Takato T, Hoshi K.  Ear Cartilage Reconstruction Combining Induced Pluripotent Stem Cell-Derived Cartilage and Three-Dimensional Shape-Memory Scaffold.  Tissue Eng Part A  2021  27(9-10):604-617  PubMed ID: 32883178   DOI: 10.1089/ten.TEA.2020.0106
20011  Chikae S, Kubota A, Nakamura H, Oda A, Yamanaka A, Akagi T, Akashi M.  Bioprinting 3D human cardiac tissue chips using the pin type printer "Microscopic painting device" and analysis for cardiotoxicity.  Biomed Mater  2021    PubMed ID: 33445157   DOI: 10.1088/1748-605X/abdbde
20102  Murayama MA, Arimitsu N, Shimizu J, Fujiwara N, Takai K, Okada Y, Hirotsu C, Takada E, Suzuki T, Suzuki N.  Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation.  Exp Anim  2021    PubMed ID: 33828024   DOI: 10.1538/expanim.21-0008
14063  Yokoyama J, Miyagawa S, Akagi T, Akashi M, Sawa Y.  Human induced pluripotent stem cell-derived three-dimensional cardiomyocyte tissues ameliorate the rat ischemic myocardium by remodeling the extracellular matrix and cardiac protein phenotype  PLoS One  2021  16(3):e0245571  PubMed ID: 33720933   DOI: 10.1371/journal.pone.0245571
20162  Hirai T, Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Koizumi N, Utoguchi N, Mizuguchi H, Sato Y.  A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells.  Sci Rep  2021  11(1)11407  PubMed ID: 34075124   DOI: 10.1038/s41598-021-90928-7
20185  Motosugi N, Okada C, Sugiyama A, Kawasaki T, Kimura M, Shiina T, Umezawa A, Akutsu H, Fukuda A.  Deletion of lncRNA XACT does not change expression dosage of X-linked genes, but affects differentiation potential in hPSCs.  Cell Rep  2021  35(10):109222  PubMed ID: 34107248   DOI: 10.1016/j.celrep.2021.109222
20193  Iseoka H, Miyagawa S, Sakai Y, Sawa Y.  Cardiac fibrosis models using human induced pluripotent stem cell-derived cardiac tissues allow anti-fibrotic drug screening in vitro.  Stem Cell Res  2021  54:102420  PubMed ID: 34126557   DOI: 10.1016/j.scr.2021.102420
20319  Takeda M, Ito E, Minami K, Harada A, Mochizuki-Oda N, Sawa Y, Miyagawa S.  Elimination of residual undifferentiated induced pluripotent stem cells (iPSCs) using irradiation for safe clinical applications of iPSC-derived cardiomyocytes.  Biochem Biophys Res Commun  2021  574:91-96  PubMed ID: 34450429   DOI: 10.1016/j.bbrc.2021.08.065
20325  Tsuji K, Yamada S, Hirai K, Asakura H, Kanda Y.  Development of alveolar and airway cells from human iPS cells: toward SARS-CoV-2 research and drug toxicity testing.  J Toxicol Sci  2021  46(9):425-435  PubMed ID: 34470994   DOI: 10.2131/jts.46.425
20329  Tadano K, Miyagawa S, Takeda M, Tsukamoto Y, Kazusa K, Takamatsu K, Akashi M, Sawa Y.  Cardiotoxicity assessment using 3D vascularized cardiac tissue consisting of human iPSC-derived cardiomyocytes and fibroblasts.  Mol Ther Methods Clin Dev  2021  22:338-349  PubMed ID: 34514026   DOI: 10.1016/j.omtm.2021.05.007
15511  Taku Futenma, Yasunori Akiyama, Sho Tanaka, Masaki Honda, Taku Toriumi  Epithelial Cell Differentiation from Human Induced Pluripotent Stem Cells Using a Single-Cell Culture Method  Journal of Hard Tissue Biology  2021  30:151-160    DOI: 10.2485/jhtb.30.151
16068  Ota A, Hyon SH, Sumi S, Matsumura K.  Gene expression analysis of human induced pluripotent stem cells cryopreserved by vitrification using StemCell Keep  Biochem Biophys Rep  2021  28:101172  PubMed ID: 34825070   DOI: 10.1016/j.bbrep.2021.101172
4117  Csobonyeiova M, Polak S, Danisovic L.  Generation of Pancreatic β-cells From iPSCs and their Potential for Type 1 Diabetes Mellitus Replacement Therapy and Modelling.  Exp. Clin. Endocrinol. Diabetes  2020    PubMed ID: 30114722   DOI: 10.1055/a-0661-5873
11778  Tateno H, Hiemori K, Minoshima F, Kiyoi K, Matoba K, Katayama J, Kumada Y.  Oriented immobilization of rBC2LCN lectin for highly sensitive detection of human pluripotent stem cells using cell culture supernatants.  J. Biosci. Bioeng.  2020  129(2):215-222.  PubMed ID: 31473147   DOI: 10.1016/j.jbiosc.2019.08.003
11938  Hashida A, Uemura T, Kino-Oka M.  Kinetics on aggregate behaviors of human induced pluripotent stem cells in static suspension and rotating flow cultures.  J. Biosci. Bioeng.  2020    PubMed ID: 31826834   DOI: 10.1016/j.jbiosc.2019.11.005
12023  Nishimoto S, Mizuno T, Takahashi K, Nagano F, Yuzawa Y, Nishiyama A, Osafune K, Hitomi H, Nagamatsu T.  CD140b and CD73 are markers for human induced pluripotent stem cell-derived erythropoietin-producing cells.  FEBS Open Bio  2020    PubMed ID: 31977161   DOI: 10.1002/2211-5463.12800
12042  Ninomiya H, Intoh A, Ishimine H, Onuma Y, Ito Y, Michiue T, Tazaki A, Kato M.  Application of a human mesoderm tissue elongation system in vitro derived from human induced pluripotent stem cells to risk assessment for teratogenic chemicals.  Chemosphere  2020    PubMed ID: 32092576   DOI: 10.1016/j.chemosphere.2020.126124
12075  Sasano Y, Fukumoto K, Tsukamoto Y, Akagi T, Akashi M.  Construction of 3D cardiac tissue with synchronous powerful beating using human cardiomyocytes from human iPS cells prepared by a convenient differentiation method.  J. Biosci. Bioeng.  2020    PubMed ID: 32151485   DOI: 10.1016/j.jbiosc.2020.01.001
12108  Tsukamoto Y, Akagi T, Akashi M.  Vascularized cardiac tissue construction with orientation by layer-by-layer method and 3D printer.  Sci Rep  2020    PubMed ID: 32218447   DOI: 10.1038/s41598-020-59371-y
13105  Takayama Y, Kushige H, Akagi Y, Suzuki Y, Kumagai Y, Kida YS.  Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.  Sci Rep  2020    PubMed ID: 32528170   DOI: 10.1038/s41598-020-66303-3
13247  Shimbo E, Nukuzuma S, Tagawa YI.  Human iPS cell-derived astrocytes support efficient replication of progressive multifocal leukoencephalopathy-type JC polyomavirus.  Biochem Biophys Res Commun  2020    PubMed ID: 33008586   DOI: 10.1016/j.bbrc.2020.09.117
13619  Noninvasive optical coherence tomography imaging of three-dimensional cardiac tissues derived from human induced pluripotent stem cells  Noninvasive optical coherence tomography imaging of three-dimensional cardiac tissues derived from human induced pluripotent stem cells  J Tissue Eng Regen Med  2020  14(10):1384-1393  PubMed ID: 32593199   DOI: 10.1002/term.3092
13637  Ikeda M, Akagi T, Nagao M, Akashi M.  Formulation Stability of Amphiphilic Poly(γ-Glutamic Acid) Nanoparticle and Evaluation of Cardiotoxicity of NPs With Human iPSC-Derived 3D-Cardiomyocyte Tissues  J Pharm Sci  2020  109(10):2969-2974  PubMed ID: 32070703   DOI: 10.1016/j.xphs.2020.01.032
13641  Tsukamoto Y, Akagi T, Akashi M.  Supersensitive Layer-by-Layer 3D Cardiac Tissues Fabricated on a Collagen Culture Vessel Using Human-Induced Pluripotent Stem Cells  Tissue Eng Part C Methods  2020  26(9):493-502  PubMed ID: 32873187   DOI: 10.1089/ten.TEC.2020.0195
13854  Samura T, Miyagawa S, Kawamura T, Fukushima S, Yokoyama JY, Takeda M, Harada A, Ohashi F, Sato-Nishiuchi R, Toyofuku T, Toda K, Sekiguchi K, Sawa Y.  Laminin-221 Enhances Therapeutic Effects of Human-Induced Pluripotent Stem Cell-Derived 3-Dimensional Engineered Cardiac Tissue Transplantation in a Rat Ischemic Cardiomyopathy Model  J Am Heart Assoc  2020  9(16):e015841  PubMed ID: 32783519   DOI: 10.1161/JAHA.119.015841
13918  Genki N. Kanda, Taku Tsuzuki, Motoki Terada, Noriko Sakai, Naohiro Motozawa, Tomohiro Masuda, Mitsuhiro Nishida, Chihaya T. Watanabe, Tatsuki Higashi, Shuhei A. Horiguchi, Taku Kudo, Motohisa Kamei, Genshiro A. Sunagawa, Kenji Matsukuma, Takeshi Sakurada, Yosuke Ozawa, Masayo Takahashi, Koichi Takahashi, Tohru Natsume  Robotic Search for Optimal Cell Culture in Regenerative Medicine  bioRXiv  2020      DOI: 10.1101/2020.11.25.392936
11235  Yoda K, Ohnuki Y, Kurosawa H.  Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions.  J. Biosci. Bioeng.  2019  127(3):381-387  PubMed ID: 30292671   DOI: 10.1016/j.jbiosc.2018.09.002
11400  Konagaya S, Iwata H.  Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells.  Sci Rep  2019    PubMed ID: 30679498   DOI: 10.1038/s41598-018-36606-7
11424  Dion J, Minoshima F, Saito S, Kiyoi K, Hasehira K, Tateno H.  Photoactivable elimination of tumorigenic human induced pluripotent stem cells using a lectin-doxorubicin prodrug conjugate.  Chembiochem  2019    PubMed ID: 30737871   DOI: 10.1002/cbic.201900086
11482  Matsumoto E, Koide N, Hanzawa H, Kiyama M, Ohta M, Kuwabara J, Takeda S, Takahashi M.  Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine.  PLoS ONE  2019    PubMed ID: 30865653   DOI: 10.1371/journal.pone.0212369
4572  Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y.  SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation.  Nat Commun  2019  10(1):2175  PubMed ID: 31092818   DOI: 10.1038/s41467-019-09511-4
11600  Miura N, Ishihara Y, Miura Y, Kimoto M, Miura K.  miR-520d-5p can reduce the mutations in hepatoma cancer cells and iPSCs-derivatives.  BMC Cancer  2019    PubMed ID: 31202279   DOI: 10.1186/s12885-019-5786-y
11732  Chikae S, Kubota A, Nakamura H, Oda A, Yamanaka A, Akagi T, Akashi M.  Three-dimensional bioprinting human cardiac tissue chips of using a painting needle method.  Biotechnol. Bioeng.  2019    PubMed ID: 31369146   DOI: 10.1002/bit.27126
11735  Umemura Y, Maki I, Tsuchiya Y, Koike N, Yagita K.  Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells.  J. Biol. Rhythms  2019    PubMed ID: 31368392   DOI: 10.1177/0748730419865436
11829  Sato M, Shiba N, Miyazaki D, Shiba Y, Echigoya Y, Yokota T, Takizawa H, Aoki Y, Takeda S, Nakamura A.  Amelioration of intracellular Ca2+ regulation by exon-45 skipping in Duchenne muscular dystrophy-induced pluripotent stem cell-derived cardiomyocytes.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31585729   DOI: 10.1016/j.bbrc.2019.09.095
6890  Yamada S, Kanda Y.  Retinoic acid promotes barrier functions in human iPSC-derived intestinal epithelial monolayers.  J Pharmacol Sci  2019  140(4):337-344  PubMed ID: 31399314   DOI: 10.1016/j.jphs.2019.06.012
10388  Ken Tomochika, Nagisa Arimitsu, Masanori A. Murayama, Chieko Hirotsu, Kazufumi Nagata, Kenji Takai, Jun Shimizu, Tsutomu Akazawa, Yoshiaki Torii, Tasuku Umehara, Masahiro Iinuma, Hisateru Niki, Noboru Suzuki  Transplantation of Human iPS Cell-Derived Neural Cells with an Artificial Nerve Conduit Leads to Cellular Retention in the Transplanted Area and Improves Motor Function in a Mouse Spinal Cord Injury Model  Journal of St. Marianna University  2019  10:27-37    DOI: 10.17264/stmarieng.10.27
12314  Kanji Hori, Junichi Kuwabara, Yuji Tanaka, Mitsuhiro Nishida, Naoshi Koide, Masayo Takahashi  A simple and static preservation system for shipping retinal pigment epithelium cell sheets  J Tissue Eng Regen Med  2019  13(3):459-468  PubMed ID: 30644171   DOI: 10.1002/term.2805
12358  Ito E, Miyagawa S, Takeda M, Kawamura A, Harada A, Iseoka H, Yajima S, Sougawa N, Mochizuki-Oda N, Yasuda S, Sato Y, Sawa Y.  Tumorigenicity assay essential for facilitating safety studies of hiPSC-derived cardiomyocytes for clinical application  Sci Rep  2019  9(1):1881  PubMed ID: 30760836   DOI: 10.1038/s41598-018-38325-5
12369  Kamao H, Miki A, Kiryu J.  Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activat  J Ophthalmol  2019  2019:7189241  PubMed ID: 31016039   DOI: 10.1155/2019/7189241
16886  Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, Shimmura S, Sugita S.  Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells  Stem Cells Dev  2019  28(1):28-43  PubMed ID: 30251915   DOI: 10.1089/scd.2018.0058
10308  Iseoka H, Miyagawa S, Fukushima S, Saito A, Masuda S, Yajima S, Ito E, Sougawa N, Takeda M, Harada A, Lee JK, Sawa Y.  Pivotal Role of Non-cardiomyocytes in Electromechanical and Therapeutic Potential of Induced Pluripotent Stem Cell-Derived Engineered Cardiac Tissue.  Tissue Eng Part A  2018  24:287-300  PubMed ID: 28498040   DOI: 10.1089/ten.TEA.2016.0535
10737  Kato Y, Kim MH, Kino-Oka M.  Comparison of growth kinetics between static and dynamic cultures of human induced pluripotent stem cells.  J. Biosci. Bioeng.  2018  125(6):736-740  PubMed ID: 29398548   DOI: 10.1016/j.jbiosc.2018.01.002
11171  Yamada S, Kubo Y, Yamazaki D, Sekino Y, Nomura Y, Yoshida S, Kanda Y.  Tributyltin Inhibits Neural Induction of Human Induced Pluripotent Stem Cells.  Sci Rep  2018  8(1):12155  PubMed ID: 30108368   DOI: 10.1038/s41598-018-30615-2
11234  Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y.  Tumorigenicity-associated characteristics of human iPS cell lines.  PLoS ONE  2018    PubMed ID: 30286143   DOI: 10.1371/journal.pone.0205022
11325  Yamada S, Yamazaki D, Kanda Y.  5-Fluorouracil inhibits neural differentiation via Mfn1/2 reduction in human induced pluripotent stem cells.  J Toxicol Sci  2018    PubMed ID: 30518710   DOI: 10.2131/jts.43.727
4069  Tadashi Kosawada, Email author Keita, Ohnishi, Hiroaki Satoh, Zhong-gang Feng, Kaoru Goto  Novel methods to apply micro dynamic stimulations on cultured adhesive cells and its application in constructing gel-embedded three-dimensional neuronal structures differentiated from human iPS cells  Microsystem Technologies  2018  24:625–638    DOI: 10.1007/s00542-017-3399-4
4113  Shoji M, Minato H, Ogaki S, Seki M, Suzuki Y, Kume S, Kuzuhara T.  Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells.  PLoS ONE  2018    PubMed ID: 30048506   DOI: 10.1371/journal.pone.0201239
4116  Uto S, Nishizawa S, Hikita A, Takato T, Hoshi K.  Application of induced pluripotent stem cells for cartilage regeneration in CLAWN miniature pig osteochondral replacement model.  Regen Ther  2018    PubMed ID: 30525076   DOI: 10.1016/j.reth.2018.06.003
4463  Masuda S, Matsuura K, Shimizu T.  Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture.  Regen Ther  2018  9:1-9  PubMed ID: 30525069   DOI: 10.1016/j.reth.2018.06.004
4464  Takeda M, Miyagawa S, Fukushima S, Saito A, Ito E, Harada A, Matsuura R, Iseoka H, Sougawa N, Mochizuki-Oda N, Matsusaki M, Akashi M, Sawa Y.  Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells.  Tissue Eng Part C Methods  2018  24(1):56-67  PubMed ID: 28967302   DOI: 10.1089/ten.TEC.2017.0247
5773  Kim MH, Matsubara Y, Fujinaga Y, Kino-Oka M.  A Simple and Robust Method for Culturing Human-Induced Pluripotent Stem Cells in an Undifferentiated State Using Botulinum Hemagglutinin.  Biotechnol J  2018  13(2)  PubMed ID: 29027750   DOI: 10.1002/biot.201700384
5777  Nakashima Y, Miyagi-Shiohira C, Noguchi H2, Omasa T.  Atorvastatin Inhibits the HIF1α-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells.  Mol Ther  2018  26(7):1715-1734  PubMed ID: 29929789   DOI: 10.1016/j.ymthe.2018.06.005
5799  Yasuda SY1, Ikeda T1,Shahsavarani H, Yoshida N, Nayer B, Hino M, Vartak-Sharma N, Suemori H, Hasegawa K.  Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells.  Nat Biomed Eng  2018  2(3):173-182  PubMed ID: 31015717   DOI: 10.1038/s41551-018-0200-7
5800  Sakai T et al  Phenotypic Screening Using Patient-Derived Induced Pluripotent Stem Cells Identified Pyr3 as a Candidate Compound for the Treatment of Infantile Hypertrophic Cardiomyopathy.  Int Heart J  2018  59(5):1096-1105  PubMed ID: 30101858   DOI: 10.1536/ihj.17-730
14053  Yamada S, Yamazaki D, Kanda Y.  Silver nanoparticles inhibit neural induction in human induced pluripotent stem cells  Nanotoxicology  2018  12(8):836-846  PubMed ID: 29902946   DOI: 10.1080/17435390.2018.1481238
7038  Liu L, Kamei KI, Yoshioka M, Nakajima M, Li J, Fujimoto N, Terada S, Tokunaga Y, Koyama Y, Sato H, Hasegawa K, Nakatsuji N, Chen Y.  Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells.  Biomaterials  2017  124:47-54  PubMed ID: 28187394   DOI: 10.1016/j.biomaterials.2017.01.039
9814  Suga M, Tachikawa S, Tateyama D, Ohnuma K, Furue MK.  Imaging-cytometry revealed spatial heterogeneities of marker expression in undifferentiated human pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2017  53:83-91  PubMed ID: 27573412   DOI: 10.1007/s11626-016-0084-3
10071  Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, Takahashi M.  Evaluation of the Surgical Device and Procedure for Extracellular Matrix-Scaffold-Supported Human iPSC-Derived Retinal Pigment Epithelium Cell Sheet Transplantation.  Invest. Ophthalmol. Vis. Sci.  2017  58:211-220  PubMed ID: 28114582   DOI: 10.1167/iovs.16-19778
10426  Tateno H, Saito S.  Engineering of a Potent Recombinant Lectin-Toxin Fusion Protein to Eliminate Human Pluripotent Stem Cells.  Molecules  2017  22:  PubMed ID: 28698527   DOI: 10.3390/molecules22071151
10503  Suzuki T, Shimizu Y, Furuhata E, Maeda S, Kishima M, Nishimura H, Enomoto S, Hayashizaki Y, Suzuki H.  RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells.  Blood Adv  2017  1:1699-1711  PubMed ID: 29296817   DOI: 10.1182/bloodadvances.2017005710
10933    Impairment of the transition from proliferative stage to prehypertrophic stage in chondrogenic differentiation of human induced pluripotent stem cells harboring the causative mutation of achondroplasia in fibroblast growth factor receptor 3  Regenerative Therapy  2017  6, 15-20  PubMed ID: 30271835   DOI: 10.1016/j.reth.2016.11.002
5765  Tateno H et al  Development of a practical sandwich assay to detect human pluripotent stem cells using cell culture media.  Regen Ther  2017  6:1-8  PubMed ID: 30271833   DOI: 10.1016/j.reth.2016.12.002
5767  Ito M, Sun S, Fukuhara T, Suzuki R, Tamai M, Yamauchi T, Nakashima K, Tagawa Y, Okazaki S, Matsuura Y, Wakita T, Suzuki T.  Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation.  Oncotarget  2017  8(33):53899-53915  PubMed ID: 28903311   DOI: 10.18632/oncotarget.19108
5768  Alsayegh K, Matsuura K, Sekine H, Shimizu T.  Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue.  Sci Rep  2017  7:45577  PubMed ID: 28361959   DOI: 10.1038/srep45577
5774  Miyagoe-Suzuki Y, Nishiyama T, Nakamura M, Narita A, Takemura F, Masuda S, Minami N, Murayama K, Komaki H, Goto YI, Takeda S.  Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status.  Stem Cells Int  2017  2017:7906843  PubMed ID: 28491099   DOI: 10.1155/2017/7906843
5795  Shigeru Yamada Yusuke Kubo Daiju Yamazaki Yuko Sekino and Yasunari Kandaa  Chlorpyrifos inhibits neural induction via Mfn1-mediated mitochondrial dysfunction in human induced pluripotent stem cells  Sci Rep.   2017  7:40925.  PubMed ID: 28112198   DOI: 10.1038/srep40925
5796  Komae H, Sekine H, Dobashi I, Matsuura K, Ono M, Okano T, Shimizu T.  Three-dimensional functional human myocardial tissues fabricated from induced pluripotent stem cells.  J Tissue Eng Regen Med  2017  11(3):926-935  PubMed ID: 25628251   DOI: 10.1002/term.1995
5797  Arauchi A, Matsuura K, Shimizu T, Okano T.  Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture.  Front Endocrinol (Lausanne)  2017  8:103  PubMed ID: 28588551   DOI: 10.3389/fendo.2017.00103
5798  Mihara Y, Matsuura K, Sakamoto Y, Okano T, Kokudo N, Shimizu T.  Production of pancreatic progenitor cells from human induced pluripotent stem cells using a three-dimensional suspension bioreactor system.  J Tissue Eng Regen Med  2017  11(11):3193-3201  PubMed ID: 28317340   DOI: 10.1002/term.2228
7047  Numaga-Tomita T, Kitajima N, Kuroda T, Nishimura A, Miyano K, Yasuda S, Kuwahara K, Sato Y, Ide T, Birnbaumer L, Sumimoto H, Mori Y, Nishida M.  TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis.  Sci Rep  2016  6:39383  PubMed ID: 27991560   DOI: 10.1038/srep39383
7080  Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.  Co-ordinated ocular development from human iPS cells and recovery of corneal function.  Nature  2016  531:376-80  PubMed ID: 26958835   DOI: 10.1038/nature17000
7083  Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.  TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.  Sci Rep  2016  6:21747  PubMed ID: 26888607   DOI: 10.1038/srep21747
9539  Amano Y, Nishiguchi A, Matsusaki M, Iseoka H, Miyagawa S, Sawa Y, Seo M, Yamaguchi T, Akashi M.  Development of vascularized iPSC derived 3D-cardiomyocyte tissues by filtration Layer-by-Layer technique and their application for pharmaceutical assays.  Acta Biomater  2016  33:110-21  PubMed ID: 26821339   DOI: 10.1016/j.actbio.2016.01.033
9674  Yamada S, Asanagi M, Hirata N, Itagaki H, Sekino Y, Kanda Y.  Tributyltin induces mitochondrial fission through Mfn1 degradation in human induced pluripotent stem cells.  Toxicol In Vitro  2016  34:257-63  PubMed ID: 27133438   DOI: 10.1016/j.tiv.2016.04.013
9838  Tomotsune D, Hirashima K, Fujii M, Yue F, Matsumoto K, Takizawa-Shirasawa S, Yokoyama T, Sasaki K.  Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment.  PLoS ONE  2016  11:e0162693  PubMed ID: 27632182   DOI: 10.1371/journal.pone.0162693
10891    Human iPS cell derived neural cell sheets exhibit mature neural and extendable scaffold functions and promote recovery in injured mouse spinal cords  J Stem Cell Res Med  2016  1: 41-47    DOI: 10.15761/JSCRM.1000107
7271  Fujiwara N, Shimizu J, Takai K, Arimitsu N, Ueda Y, Wakisaka S, Suzuki T, Suzuki N.  Cellular and molecular mechanisms of the restoration of human APP transgenic mouse cognitive dysfunction after transplant of human iPS cell-derived neural cells.  Exp. Neurol.  2015  271:423-31  PubMed ID: 26196079   DOI: 10.1016/j.expneurol.2015.07.008
7518  Kagawa Y, Matsuura K, Shimizu T, Tsuneda S.  Direct measurement of local dissolved oxygen concentration spatial profiles in a cell culture environment.  Biotechnol. Bioeng.  2015  112:1263-74  PubMed ID: 25565074   DOI: 10.1002/bit.25531
7542  M Iinuma, T Umehara, N Arimitsu, J Shimizu, H Misawa, K Takai, N Fujiwara, A Fujii, Y Ueda, S Wakisaka, T Suzuki, C Hirotsu, M Beppu, N Suzuki  Induction of neural cells with spinal motoneuron phenotype from human iPS cells and the trans-plantation to totally transected spinal cords in mice.  Inflammation and Regeneration  2015  35:154-163    DOI: 10.2492/inflammregen.35.154
7562  Takuya Kuroda, Satoshi Yasuda, Satoko Matsuyama a, Keiko Tano, Shinji Kusakawa, Yoshiki Sawa, Shin Kawamata, Yoji Sato  Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells.  Regenerative Therapy  2015  2:17-23  PubMed ID: 31245455   DOI: 10.1016/j.reth.2015.08.001
5449  Tateno H, Onuma Y, Ito Y, Minoshima F, Saito S, Shimizu M, Aiki Y, Asashima M, Hirabayashi J.  Elimination of tumorigenic human pluripotent stem cells by a recombinant lectin-toxin fusion protein.  Stem Cell Reports  2015  4(5):811-20  PubMed ID: 25866158   DOI: 10.1016/j.stemcr.2015.02.016
5729  Matsuura K, Kodama F, Sugiyama K, Shimizu T, Hagiwara N, Okano T.  Elimination of remaining undifferentiated induced pluripotent stem cells in the process of human cardiac cell sheet fabrication using a methionine-free culture condition.  Tissue Eng Part C Methods  2015  21(3):330-8  PubMed ID: 25245976   DOI: 10.1089/ten.TEC.2014.0198
5752  Onuma Y, Higuchi K, Aiki Y, Shu Y, Asada M, Asashima M, Suzuki M, Imamura T, Ito Y.  A stable chimeric fibroblast growth factor (FGF) can successfully replace basic FGF in human pluripotent stem cell culture.  PLoS One  2015  10(4):e0118931  PubMed ID: 25850016   DOI: 10.1371/journal.pone.0118931
5753  Komatsu M, Konagaya S, Egawa EY, Iwata H.  Maturation of human iPS cell-derived dopamine neuron precursors in alginate-Ca(2+) hydrogel.  Biochim Biophys Acta  2015  1850(9):1669-75  PubMed ID: 25952075   DOI: 10.1016/j.bbagen.2015.04.011
5755  Konagaya S, Iwata H.  Microencapsulation of dopamine neurons derived from human induced pluripotent stem cells.  Biochim Biophys Acta  2015  1850(1):22-32  PubMed ID: 25281770   DOI: 10.1016/j.bbagen.2014.09.025
5757  Haraguchi Y, Matsuura K, Shimizu T, Yamato M, Okano T.  Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering.  J Tissue Eng Regen Med  2015  9(12):1363-75  PubMed ID: 23728860   DOI: 10.1002/term.1761
5788  Toriumi T, Takayama N, Murakami M, Sato M, Yuguchi M, Yamazaki Y, Eto K, Otsu M, Nakauchi H, Shirakawa T, Isokawa K, Honda MJ.  Characterization of mesenchymal progenitor cells in the crown and root pulp of primary teeth.  Biomed Res  2015  36(1):31-45  PubMed ID: 25749149   DOI: 10.2220/biomedres.36.31
5789  Takaki Shiratsuchi, Hiroko Misawa, Asako Saito, Jun Shimizu, Masahiro Iinuma, Naruyoshi Fujiwara, Kenji Takai, Nagisa Arimitsu, Yuji Ueda, Sueshige Wakisaka, Tomoko Suzuki, Moroe Beppu, Noboru Suzuki  Sonic Hedgehog Supplementation Rapidly induces Myogenesis in Human Induced Pluripotent Stem Cells  Journal of St. Marianna University  2015  6:225-233    DOI: 10.17264/stmarieng.6.225
5790  Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.  Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.  Sci Rep  2015  5:16647  PubMed ID: 26573336   DOI: 10.1038/srep16647
5792  Nagaoka M, Kobayashi M, Kawai C, Mallanna SK, Duncan SA.  Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells.  PLoS One  2015  10(8):e0136350  PubMed ID: 26308339   DOI: 10.1371/journal.pone.0136350
5793  Arai D, Hayakawa K, Ohgane J, Hirosawa M, Nakao Y, Tanaka S, Shiota K.  An epigenetic regulatory element of the Nodal gene in the mouse and human genomes.  Mech Dev  2015  136:143-54  PubMed ID: 25528267   DOI: 10.1016/j.mod.2014.12.003
5794  Mitsui K, Ide K, Takayama A, Wada T, Irie R, Kosai K.  Conditionally replicating adenovirus prevents pluripotent stem cell-derived teratoma by specifically eliminating undifferentiated cells.  Mol Ther Methods Clin Dev  2015  2:15026  PubMed ID: 26269798   DOI: 10.1038/mtm.2015.26
15253  Liu CY, Matsusaki M, Akashi M.  Control of Cell-Cell Distance and Cell Densities in Millimeter-Sized 3D Tissues Constructed by Collagen Nanofiber Coating Techniques  ACS Biomater Sci Eng  2015  1(8):639-645  PubMed ID: 33435087   DOI: 10.1021/acsbiomaterials.5b00015
5725  Imaizumi K, Nishishita N, Muramatsu M, Yamamoto T, Takenaka C, Kawamata S, Kobayashi K, Nishikawa S, Akuta T.  A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol.  PLoS One  2014  9(2):e88696  PubMed ID: 24533137   DOI: 10.1371/journal.pone.0088696
5732  Tokunaga K, Saitoh N, Goldberg IG, Sakamoto C, Yasuda Y, Yoshida Y, Yamanaka S, Nakao M.  Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells.  Sci Rep  2014  4:6996  PubMed ID: 25385348   DOI: 10.1038/srep06996
5733  Ohnuma K, Fujiki A, Yanagihara K, Tachikawa S, Hayashi Y, Ito Y, Onuma Y, Chan T, Michiue T, Furue MK, Asashima M  Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.  Sci Rep  2014  4:4646  PubMed ID: 24721898   DOI: 10.1038/srep04646
5734  Tateno H, Onuma Y, Ito Y, Hiemori K, Aiki Y, Shimizu M, Higuchi K, Fukuda M, Warashina M, Honda S, Asashima M, Hirabayashi J.  A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells.  Sci Rep  2014  4:4069  PubMed ID: 24518842   DOI: 10.1038/srep04069
5735  Yamada R, Hattori K, Tachikawa S, Tagaya M, Sasaki T, Sugiura S, Kanamori T, Ohnuma K.  Control of adhesion of human induced pluripotent stem cells to plasma-patterned polydimethylsiloxane coated with vitronectin and γ-globulin.  J Biosci Bioeng  2014  118(3):315-22  PubMed ID: 24656306   DOI: 10.1016/j.jbiosc.2014.02.009
5741  Tano K, Yasuda S, Kuroda T, Saito H, Umezawa A, Sato Y.  A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system.  PLoS One  2014  9(10):e110496  PubMed ID: 25347300   DOI: 10.1371/journal.pone.0110496
5745  Luo L, Kawakatsu M, Guo CW, Urata Y, Huang WJ, Ali H, Doi H, Kitajima Y, Tanaka T, Goto S, Ono Y, Xin HB, Hamano K, Li TS.  Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.  Sci Rep  2014  4:3779  PubMed ID: 24445363   DOI: 10.1038/srep03779
5785  Kamao H, Mandai M, Okamoto S, Sakai N, Suga A, Sugita S, Kiryu J, Takahashi M  Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application.  Stem Cell Reports  2014  2(2):205-18  PubMed ID: 24527394   DOI: 10.1016/j.stemcr.2013.12.007
5786  Sekine K, Kagawa Y, Maeyama E, Ota H, Haraguchi Y, Matsuura K, Shimizu T.  Oxygen consumption of human heart cells in monolayer culture.  Biochem Biophys Res Commun  2014  452(3):834-9.   PubMed ID: 25218502   DOI: 10.1016/j.bbrc.2014.09.018
5787  Kamao H, Mandai M, Wakamiya S, Ishida J, Goto K, Ono T, Suda T, Takahashi M, Kiryu J.  Objective evaluation of the degree of pigmentation in human induced pluripotent stem cell-derived RPE.  Invest Ophthalmol Vis Sci  2014  55(12):8309-18.  PubMed ID: 25389202   DOI: 10.1167/iovs.14-14694
13757  Ishihara Y, Tsuno S, Kuwamoto S, Yamashita T, Endo Y, Hasegawa J, Miura N.  Hsa-miR-520d converts fibroblasts into CD105+ populations  Drugs R D  2014  14(4):253-64.  PubMed ID: 25303886   DOI: 10.1007/s40268-014-0064-6
5710  Fujiwara N, Shimizu J, Takai K, Arimitsu N, Saito A, Kono T, Umehara T, Ueda Y, Wakisaka S, Suzuki T, Suzuki N.  Restoration of spatial memory dysfunction of human APP transgenic mice by transplantation of neuronal precursors derived from human iPS cells.  Neurosci Lett  2013  557 Pt B:129-34  PubMed ID: 24466594  
5714  Onuma Y, Tateno H, Hirabayashi J, Ito Y, Asashima M.  rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells.  Biochem Biophys Res Commun  2013  431(3):524-9  PubMed ID: 23321312   DOI: 10.1016/j.bbrc.2013.01.025
5722  Guo CW, Kawakatsu M, Idemitsu M, Urata Y, Goto S, Ono Y, Hamano K, Li TS.  Culture under low physiological oxygen conditions improves the stemness and quality of induced pluripotent stem cells.  J Cell Physiol  2013  228(11):2159-66  PubMed ID: 23589166   DOI: 10.1002/jcp.24389
5783  Kanemura H, Go MJ, Nishishita N, Sakai N, Kamao H, Sato Y, Takahashi M, Kawamata S.  Pigment epithelium-derived factor secreted from retinal pigment epithelium facilitates apoptotic cell death of iPSC.  Sci Rep  2013  3:2334  PubMed ID: 23903667   DOI: 10.1038/srep02334
5784  Hiroyuki KAMAO, Junko ISHIDA, Taiji SUDA, Junichi KIRYU  Generation of retinal pigment epithelium from human induced pluripotent stem cells showed polarized secretion of VEGF and PEDF.  Kawasaki Medical Journal   2013  39:155-162   
7597  Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K.  Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells.  Biochem. Biophys. Res. Commun.  2012  417:836-41  PubMed ID: 22206673   DOI: 10.1016/j.bbrc.2011.12.052
5702  Hayashi R, Ishikawa Y, Ito M, Kageyama T, Takashiba K, Fujioka T, Tsujikawa M, Miyoshi H, Yamato M, Nakamura Y, Nishida K.  Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.  PLoS One  2012  7(9):e45435  PubMed ID: 23029008   DOI: 10.1371/journal.pone.0045435
5706  Otsuji TG, Kurose Y, Suemori H, Tada M, Nakatsuji N.  Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes.  PLoS One  2012  7(9):e45010  PubMed ID: 22984602   DOI: 10.1371/journal.pone.0045010
5781  Matsuura K, Wada M, Shimizu T, Haraguchi Y, Sato F, Sugiyama K, Konishi K, Shiba Y, Ichikawa H, Tachibana A, Ikeda U, Yamato M, Hagiwara N, Okano T.  Creation of human cardiac cell sheets using pluripotent stem cells.  Biochem Biophys Res Commun  2012  425(2):321-7  PubMed ID: 22842572   DOI: 10.1016/j.bbrc.2012.07.089
5782  Ueda Y, Fujita S, Nishigaki T, Arima Y, Iwata H.  Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells.  J Biomater Sci Polym Ed  2012  23(1-4):153-65  PubMed ID: 22133351   DOI: 10.1163/092050610X545797
7771  Ogawa S, Tokumoto Y, Miyake J, Nagamune T.  Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells.  In Vitro Cell. Dev. Biol. Anim.  2011  47:464-9  PubMed ID: 21695581   DOI: 10.1007/s11626-011-9435-2
5750  Kumazaki T, Kurata S, Matsuo T, Mitsui Y, Takahashi T.  Establishment of human induced pluripotent stem cell lines from normal fibroblast TIG-1.  Hum Cell  2011  24(2):96-103  PubMed ID: 21562774   DOI: 10.1007/s13577-011-0016-1
5780  Nishigaki T, Teramura Y, Nasu A, Takada K, Toguchida J, Iwata H.  Highly efficient cryopreservation of human induced pluripotent stem cells using a dimethyl sulfoxide-free solution.  Int J Dev Biol  2011  55(3):305-11  PubMed ID: 21710436   DOI: 10.1387/ijdb.103145tn
5779  Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S.  Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53.  Exp Hematol  2010  38(2):154-62  PubMed ID: 19922768   DOI: 10.1016/j.exphem.2009.11.003
10089  Burden ML, Burden AC.  The American Diabetes Association screening questionnaire for diabetes. Is it worthwhile in the U.K.?  Diabetes Care  1994  17:97  PubMed ID: 8112198   DOI: 10.2337/diacare.17.1.97



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