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Cell No. : Cell Name
HEV0137 :  update : 2021/03/29
CommentHuman B lymphocytes transformed by Epstein-Barr Virus. HLA A*02:01 A*24:02, B*46:01 B*67:01, C*01:02 C*07:02, DRB1*08:03 DRB1*09:01, DQB1*03:03 DQB1*06:01, DPB1*02:01 DPB1*05:01
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.(Mutat Res 2005; 588: 1-6) designated by the DEPOSITOR is required. 2) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0047.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Year of deposit 2004
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 65
Tissue peripheral blood lymphocytes
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology lymphocyte-like
HEV related information Alcohol No
Smoking Smoking
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 20% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
3601  Iwakawa M, Goto M, Noda S, Sagara M, Yamada S, Yamamoto N, Kawakami Y, Matsui Y, Miyazawa Y, Yamazaki H, Tsuji H, Ohno T, Mizoe J, Tsujii H, Imai T.  DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers.  Mutat Res  2005  588(1):1-6  PubMed ID: 16260175
3157  Ban S, Konomi C, Iwakawa M, Yamada S, Ohno T, Tsuji H, Noda S, Matui Y, Harada Y, Cologne JB, Imai T.  Radiosensitivity of peripheral blood lymphocytes obtained from patients with cancers of the breast, head and neck or cervix as determined with a micronucleus assay.  J Radiat Res  2004  5(4):535-41  PubMed ID: 15635263

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