Cell No. : Cell Name
HES0653 : KhES-1_Crx::Venus(m1-F11)
update : 2024/02/06
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| Comment | Human embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure. |
| Comment from the depositor | KhES-1 cell line, carrying Venus knocked in the Crx locus. Crx^(wt/Venus). |
| Terms and conditions | 1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . |
| Remarks | |
| Order Form |
Order Form(C-0061.pdf)
Approval Form(C-0006.pdf)
MTA(C-0062.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Eiraku, Mototsugu
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| Year of deposit |
2017
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Female
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (without L-Glutamine) + 2mM L-Glutamine + 20% KSR + 0.08mM NEAA + 0.08mM 2-Mercaptoethanol + 4ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-4 split
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| SC frequency |
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Subculture : once/3-4 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10 5 cells/60 mm dish
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| Freeze medium |
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DAP213 : MEM + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
1
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| Reference |
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6823
Kadoshima T, Sakaguchi H, Nakano T, Soen M, Ando S, Eiraku M, Sasai Y.
Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex.
Proc Natl Acad Sci U S A
2013
110(50):20284-9
PubMed ID: 24277810
DOI: 10.1073/pnas.1315710110
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6808
Nakano T, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y.
Self-formation of optic cups and storable stratified neural retina from human ESCs.
Cell Stem Cell
2012
10(6):771-785
PubMed ID: 22704518
DOI: 10.1016/j.stem.2012.05.009
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SDS_DAP(MEM)_Japanese.pdf