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Cell No. : Cell Name
HES0652 : KhES-1_Rx::Venus(VA22-N37)  update : 2021/12/21
CommentHuman embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure.
Comment from the depositorKhES-1 cell line, carrying Venus knocked in the Rx locus. Rx^(wt/Venus).
Terms and conditions1) BIOLOGICAL RESOURCE is available only for academic research by not-for-profit organization. 2) Please access "Human ES cell project information disclosure page". 3) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Remarks
Order Form Order Form(C-0061.pdf)   Approval Form(C-0006.pdf)   MTA(C-0062.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Eiraku, Mototsugu
Year of deposit 2017
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (without L-Glutamine) + 2mM L-Glutamine + 20% KSR + 0.08mM NEAA + 0.08mM 2-Mercaptoethanol + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-4 split
SC frequency Subculture : once/3-4 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium DAP213 : MEM + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 2
User's Publication 0


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Reference
6823  Kadoshima T, Sakaguchi H, Nakano T, Soen M, Ando S, Eiraku M, Sasai Y.  Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex.  Proc Natl Acad Sci U S A  2013  110(50):20284-9  PubMed ID: 24277810
6808  Nakano T, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y.  Self-formation of optic cups and storable stratified neural retina from human ESCs.  Cell Stem Cell  2012  10(6):771-785  PubMed ID: 22704518

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