Cell No. : Cell Name
HES0008 : SEES3
update : 2021/11/18
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| Comment | Human embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure. |
| Comment from the depositor | |
| Terms and conditions | Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . |
| Remarks | |
| Order Form |
Order Form(C-0061.pdf)
MTA(C-0062.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Umezawa, Akihiro
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| Originator |
Umezawa, Akihiro
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| Year of deposit |
2011
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_1Q91
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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iPSellon + 10ng/ml human basic FGF
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| Antibiotics |
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within iPSellon （Penicillin, Streptomycin）
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Passage method |
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0.1% Dispase II
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 3-6 split
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| SC frequency |
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Subculture : once/4-6 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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4-5 x 10 5 cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| Chromosome mode |
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46XY(20)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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SDS_DAP(MEM)_Japanese.pdf