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Cell No. : Cell Name
HES0008 : SEES3  update : 2020/01/14
CommentHuman embryonic stem cell line.
Comment from the depositor
Terms and conditionsPlease read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Remarks
Order Form Order Form(C-0061.pdf)   MTA(C-0062.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2011
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives iPSellon + 10ng/ml human basic FGF
Antibiotics within iPSellon (Penicillin, Streptomycin)
Passage method 0.1% Dispase II
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-6 split
SC frequency Subculture : once/4-6 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 4-5 x 10^(5) cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Chromosome mode 46XY(20)
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 0
User's Publication 0


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