Cell No. : Cell Name
HES0005 : KhES-5
update : 2020/01/14
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| Comment | Human embryonic stem cell line. |
| Comment from the depositor | |
| Terms and conditions | 1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Nakatsuji, Norio
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| Originator |
Nakatsuji, Norio
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| Year of deposit |
2008
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| Animal |
human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/3-4 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10^(5) cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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SDS_DAP(MEM)_Japanese.pdf