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Cell No. : Cell Name
HES0005 : KhES-5  update : 2023/12/22
CommentHuman embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure.
Comment from the depositor
Terms and conditions1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Remarks
Order Form Order Form(C-0061.pdf)   Approval Form(C-0006.pdf)   MTA(C-0062.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nakatsuji, Norio
Originator Nakatsuji, Norio
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T798
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-4 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10 5 cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
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Relational File deposit infolot info
SDS_DAP(MEM)_Japanese.pdf
Reference information Reference 1
User's Publication 0


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Reference
3679  Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.  Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.  Biochem Biophys Res Commun  2006  345(3):926-32  PubMed ID: 16707099   DOI: 10.1016/j.bbrc.2006.04.135

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