Cell No. : Cell Name
HES0005 : KhES-5
update : 2020/01/14
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| Comment | Human embryonic stem cell line. |
| Comment from the depositor | |
| Terms and conditions | 1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . |
| Remarks | |
| Order Form |
Order Form(C-0061.pdf)
Approval Form(C-0006.pdf)
MTA(C-0062.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nakatsuji, Norio
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| Originator |
Nakatsuji, Norio
|
| Year of deposit |
2008
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
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| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
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| Classification |
ES
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|
Lifespan |
infinite
|
| Morphology |
ES-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/3-4 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10^(5) cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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SDS_DAP(MEM)_Japanese.pdf