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Cell No. : Cell Name
HES0003 : KhES-3  update : 2020/01/14
CommentHuman embryonic stem cell line.
Comment from the depositor
Terms and conditions1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nakatsuji, Norio
Originator Nakatsuji, Norio
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-4 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Relational File deposit infolot info
SDS_DAP(hESM)_Japanese.pdf
Reference information Reference 5
User's Publication 1


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Reference
3681  Sumi T, Tsuneyoshi N, Nakatsuji N, Suemori H.  Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc.  Oncogene  2007  26(38):5564-76  PubMed ID: 17369859
3682  Hasegawa K, Cowan AB, Nakatsuji N, Suemori H.  Efficient multicistronic expression of a transgene in human embryonic stem cells.  Stem Cells  2007  25(7):1707-12  PubMed ID: 17395772
3679  Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.  Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.  Biochem Biophys Res Commun  2006  345(3):926-32  PubMed ID: 16707099
3680  Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H.  A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.  Stem Cells  2006  24(12):2649-60  PubMed ID: 16931777
3677  Nakatsuji N.  Establishment and manipulation of monkey and human embryonic stem cell lines for biomedical research.  Ernst Schering Res Found Workshop  2005  54:15-26.  PubMed ID: 16080284

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User's Publication
9526  Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.  Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.  Nat Commun  2016  7:10351  PubMed ID: 26762480



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