Cell No. : Cell Name
HES0002 : KhES-2
update : 2024/05/28
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| Comment | Human embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure. |
| Comment from the depositor | |
| Terms and conditions | 1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . |
| Remarks | |
| Order Form |
Order Form(C-0061.pdf)
Approval Form(C-0006.pdf)
MTA(C-0062.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nakatsuji, Norio
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| Originator |
Nakatsuji, Norio
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| Year of deposit |
2008
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| Animal |
_human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Female
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_B232
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/3-4 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10 5 cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma/Acholeplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
5
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| User's Publication |
0
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| Reference |
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3681
Sumi T, Tsuneyoshi N, Nakatsuji N, Suemori H.
Apoptosis and differentiation of human embryonic stem cells induced by sustained activation of c-Myc.
Oncogene
2007
26(38):5564-76
PubMed ID: 17369859
DOI: 10.1038/sj.onc.1210353
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3682
Hasegawa K, Cowan AB, Nakatsuji N, Suemori H.
Efficient multicistronic expression of a transgene in human embryonic stem cells.
Stem Cells
2007
25(7):1707-12
PubMed ID: 17395772
DOI: 10.1634/stemcells.2006-0813
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3679
Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.
Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.
Biochem Biophys Res Commun
2006
345(3):926-32
PubMed ID: 16707099
DOI: 10.1016/j.bbrc.2006.04.135
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3680
Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H.
A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.
Stem Cells
2006
24(12):2649-60
PubMed ID: 16931777
DOI: 10.1634/stemcells.2005-0657
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3677
Nakatsuji N.
Establishment and manipulation of monkey and human embryonic stem cell lines for biomedical research.
Ernst Schering Res Found Workshop
2005
54:15-26.
PubMed ID: 16080284
DOI: 10.1007/3-540-37644-5_2
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SDS_DAP(hESM)_Japanese.pdf