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細胞材料の再培養方法 ( HPS以外・ HPS )
細胞番号 : 細胞名
HES0001 : KhES-1  update : 2023/12/22
細胞特性(Comment:英)Human embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure.
細胞特性(日)ヒト胚性幹（ES）細胞株。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
使用条件(日)1) 「ヒトES細胞プロジェクト情報公開ページ」.にアクセスし、必要な手続きをとること。 2) 「ヒトES細胞の使用に関する指針 (文部科学省) 」を遵守すること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0061.pdf)   Approval Form(C-0006.pdf)   MTA(C-0062.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0038.pdf   承諾書C-0002.pdf   同意書C-0039.pdf   締結依頼書C-0040.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Nakatsuji, Norio
樹立者 Nakatsuji, Norio
寄託日 2008
動物種 _human < Mammals
属名 Homo
種名 sapiens
性別 Female
齢 embryo
採取組織 embryonic stem cells
細胞分類 ES
細胞寿命 infinite
細胞形態 ES-like
Cellosaurus(Expasy) CVCL_B231
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
抗生物質 Free
継代方法 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
培養容器のコーティング 0.1% gelatin coated dish
継代密度 1 : 2-4 split
継代・培地交換頻度 Subculture : once/3-4 days, Medium Renewal : every day
培養最適温度 37 ℃
二酸化炭素濃度 3 %
フィーダー細胞 MEF
フィーダー細胞の処理方法 X-rays 5000R (or MMC)
フィーダー細胞の播種数 3-5 x 10 5 cells/60 mm dish
凍結培地 DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
凍結方法 Vitrification
マイコプラズマ (-)
個体識別検査 OK
画像情報
寄託時情報
ロット情報
その他の細胞情報 寄託時情報ロット情報
SDS_DAP(hESM)_Japanese.pdf
文献情報 Reference(英) 7件
Reference(日) 0件
利用者成果(英) 15件
利用者成果(日) 0件

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Reference(英)
5705  Otsuji TG, Kurose Y, Suemori H, Tada M, Nakatsuji N.  Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes.  PLoS One  2012  7(9):e45010  PubMed ID: 22984602   DOI: 10.1371/journal.pone.0045010
3678  Sakurai K, Shimoji M, Tahimic CG, Aiba K, Kawase E, Hasegawa K, Amagai Y, Suemori H, Nakatsuji N.  Efficient integration of transgenes into a defined locus in human embryonic stem cells.  Nucleic Acids Res  2010  38(7):e96  PubMed ID: 20071742   DOI: 10.1093/nar/gkp1234
3682  Hasegawa K, Cowan AB, Nakatsuji N, Suemori H.  Efficient multicistronic expression of a transgene in human embryonic stem cells.  Stem Cells  2007  25(7):1707-12  PubMed ID: 17395772   DOI: 10.1634/stemcells.2006-0813
3679  Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.  Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.  Biochem Biophys Res Commun  2006  345(3):926-32  PubMed ID: 16707099   DOI: 10.1016/j.bbrc.2006.04.135
3680  Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H.  A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.  Stem Cells  2006  24(12):2649-60  PubMed ID: 16931777   DOI: 10.1634/stemcells.2005-0657
3677  Nakatsuji N.  Establishment and manipulation of monkey and human embryonic stem cell lines for biomedical research.  Ernst Schering Res Found Workshop  2005  54:15-26.  PubMed ID: 16080284   DOI: 10.1007/3-540-37644-5_2
10895  Tsuyoshi Fujioka, Kentaro Yasuchika, Yukio Nakamura, Norio Nakatsuji, Hirofumi Suemori  A Simple and Efficient Cryopreservation Method for Primate Embryonic Stem Cells  Int J Dev Biol  2004  48(10):1149-54  PubMed ID: 15602701   DOI: 10.1387/ijdb.041852tf

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Reference(日)

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利用者成果(英)
19581  Sasaki H, Suga H, Takeuchi K, Nagata Y, Harada H, Kondo T, Ito E, Maeda S, Sakakibara M, Soen M, Miwata T, Asano T, Ozaki H, Taga S, Kuwahara A, Nakano T, Arima H, Saito R.  Subcutaneous transplantation of human embryonic stem cells-derived pituitary organoids  Front Endocrinol (Lausanne)  2023  14:1130465  PubMed ID: 36936140   DOI: 10.3389/fendo.2023.1130465
21260  Taga S, Suga H, Nakano T, Kuwahara A, Inoshita N, Kodani Y, Nagasaki H, Sato Y, Tsumura Y, Sakakibara M, Soen M, Miwata T, Ozaki H, Kano M, Watari K, Ikeda A, Yamanaka M, Takahashi Y, Kitamoto S, Kawaguchi Y, Miyata T, Kobayashi T, Sugiyama M, Onoue T, Yasuda Y, Hagiwara D, Iwama S, Tomigahara Y, Kimura T, Arima H.  Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation.  Stem Cell Reports  2023    PubMed ID: 37295423   DOI: 10.1016/j.stemcr.2023.05.002
20662  Hayashi R, Okubo T, Kudo Y, Ishikawa Y, Imaizumi T, Suzuki K, Shibata S, Katayama T, Park SJ, Young RD, Quantock AJ, Nishida K.  Generation of 3D lacrimal gland organoids from human pluripotent stem cells.  Nature  2022    PubMed ID: 35444274   DOI: 10.1038/s41586-022-04613-4
17191  Yamamoto T, Arita M, Kuroda H, Suzuki T, Kawamata S.  Improving the differentiation potential of pluripotent stem cells by optimizing culture conditions  Sci Rep  2022  12(1):14147  PubMed ID: 35986054   DOI: 10.1038/s41598-022-18400-8
21377  Shiraki N, Maruyama K, Hayashi R, Oguchi A, Murakawa Y, Katayama T, Takigawa T, Sakimoto S, Quantock AJ, Tsujikawa M, Nishida K.  PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids.  Stem Cell Reports  2022  17(2):221-230  PubMed ID: 35030319   DOI: 10.1016/j.stemcr.2021.12.009
7003  Yamamoto T, Takenaka C, Yoda Y, Oshima Y, Kagawa K, Miyajima H, Sasaki T, Kawamata S.  Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7.  Sci Rep  2018  8:241  PubMed ID: 29321579   DOI: 10.1038/s41598-017-18439-y
4274  Kano M, Suga H, Kasai T, Ozone C, Arima H.  Functional Pituitary Tissue Generation from Human Embryonic Stem Cells.  Curr Protoc Neurosci  2018    PubMed ID: 30040229   DOI: 10.1002/cpns.48
7009  Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe SI, Nishina S, Azuma N.  Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.  Sci Rep  2017  7:16757  PubMed ID: 29196712   DOI: 10.1038/s41598-017-16727-1
13931  Ota A, Matsumura K, Lee JJ, Sumi S, Hyon SH.  StemCell Keep™ Is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification  Cell Transplant  2017  26(5):773-787  PubMed ID: 27503846   DOI: 10.3727/096368916X692654
7315  Imaizumi K, Iha M, Nishishita N, Kawamata S, Nishikawa S, Akuta T.  A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.  Methods Mol. Biol.  2016  1341:15-24  PubMed ID: 26069023   DOI: 10.1007/7651_2015_211
9526  Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.  Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.  Nat Commun  2016  7:10351  PubMed ID: 26762480   DOI: 10.1038/ncomms10351
9553  Sakaguchi H, Kadoshima T, Soen M, Narii N, Ishida Y, Ohgushi M, Takahashi J, Eiraku M, Sasai Y.  Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue.  Nat Commun  2015  6:8896  PubMed ID: 26573335   DOI: 10.1038/ncomms9896
5749  Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, Ohshima Y, Kagawa K, Sasaki T, Kawamata S.  Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.  PLoS One  2015  10(6):e0129855  PubMed ID: 26115194   DOI: 10.1371/journal.pone.0129855
11943  Naoki Nishishita, Marie Muramatsu, Shin Kawamata  An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions  Am J Stem Cells  2015  4(1):38-49  PubMed ID: 25973330  
5725  Imaizumi K, Nishishita N, Muramatsu M, Yamamoto T, Takenaka C, Kawamata S, Kobayashi K, Nishikawa S, Akuta T.  A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol.  PLoS One  2014  9(2):e88696  PubMed ID: 24533137   DOI: 10.1371/journal.pone.0088696

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