Cell No. : Cell Name
HES0001 : KhES-1
update : 2021/11/18
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| Comment | Human embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure. |
| Comment from the depositor | |
| Terms and conditions | 1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells . |
| Remarks | |
| Order Form |
Order Form(C-0061.pdf)
Approval Form(C-0006.pdf)
MTA(C-0062.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nakatsuji, Norio
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| Originator |
Nakatsuji, Norio
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| Year of deposit |
2008
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| Animal |
human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Gender |
Female
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_B231
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
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| Antibiotics |
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Free
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Passage method |
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0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 2-4 split
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| SC frequency |
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Subculture : once/3-4 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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3
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10^(5) cells/60 mm dish
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| Freeze medium |
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DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
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| Freezing method |
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Vitrification
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| Mycoplasma |
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(-)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Relational File |
deposit info | lot info |
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| Reference information |
Reference |
7
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| User's Publication |
11
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| Reference |
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5705
Otsuji TG, Kurose Y, Suemori H, Tada M, Nakatsuji N.
Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes.
PLoS One
2012
7(9):e45010
PubMed ID: 22984602
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3678
Sakurai K, Shimoji M, Tahimic CG, Aiba K, Kawase E, Hasegawa K, Amagai Y, Suemori H, Nakatsuji N.
Efficient integration of transgenes into a defined locus in human embryonic stem cells.
Nucleic Acids Res
2010
38(7):e96
PubMed ID: 20071742
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3682
Hasegawa K, Cowan AB, Nakatsuji N, Suemori H.
Efficient multicistronic expression of a transgene in human embryonic stem cells.
Stem Cells
2007
25(7):1707-12
PubMed ID: 17395772
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3679
Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.
Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.
Biochem Biophys Res Commun
2006
345(3):926-32
PubMed ID: 16707099
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3680
Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H.
A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.
Stem Cells
2006
24(12):2649-60
PubMed ID: 16931777
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3677
Nakatsuji N.
Establishment and manipulation of monkey and human embryonic stem cell lines for biomedical research.
Ernst Schering Res Found Workshop
2005
54:15-26.
PubMed ID: 16080284
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10895
Tsuyoshi Fujioka, Kentaro Yasuchika, Yukio Nakamura, Norio Nakatsuji, Hirofumi Suemori
A Simple and Efficient Cryopreservation Method for Primate Embryonic Stem Cells
Int J Dev Biol
2004
48(10):1149-54
PubMed ID: 15602701
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| User's Publication |
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20662
Hayashi R, Okubo T, Kudo Y, Ishikawa Y, Imaizumi T, Suzuki K, Shibata S, Katayama T, Park SJ, Young RD, Quantock AJ, Nishida K.
Generation of 3D lacrimal gland organoids from human pluripotent stem cells.
Nature
2022
PubMed ID: 35444274
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7003
Yamamoto T, Takenaka C, Yoda Y, Oshima Y, Kagawa K, Miyajima H, Sasaki T, Kawamata S.
Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7.
Sci Rep
2018
8:241
PubMed ID: 29321579
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4274
Kano M, Suga H, Kasai T, Ozone C, Arima H.
Functional Pituitary Tissue Generation from Human Embryonic Stem Cells.
Curr Protoc Neurosci
2018
PubMed ID: 30040229
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7009
Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe SI, Nishina S, Azuma N.
Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.
Sci Rep
2017
7:16757
PubMed ID: 29196712
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13931
Ota A, Matsumura K, Lee JJ, Sumi S, Hyon SH.
StemCell Keep™ Is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification
Cell Transplant
2017
26(5):773-787
PubMed ID: 27503846
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7315
Imaizumi K, Iha M, Nishishita N, Kawamata S, Nishikawa S, Akuta T.
A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.
Methods Mol. Biol.
2016
1341:15-24
PubMed ID: 26069023
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9526
Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.
Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.
Nat Commun
2016
7:10351
PubMed ID: 26762480
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9553
Sakaguchi H, Kadoshima T, Soen M, Narii N, Ishida Y, Ohgushi M, Takahashi J, Eiraku M, Sasai Y.
Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue.
Nat Commun
2015
6:8896
PubMed ID: 26573335
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5749
Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, Ohshima Y, Kagawa K, Sasaki T, Kawamata S.
Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.
PLoS One
2015
10(6):e0129855
PubMed ID: 26115194
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11943
Naoki Nishishita, Marie Muramatsu, Shin Kawamata
An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions
Am J Stem Cells
2015
4(1):38-49
PubMed ID: 25973330
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5725
Imaizumi K, Nishishita N, Muramatsu M, Yamamoto T, Takenaka C, Kawamata S, Kobayashi K, Nishikawa S, Akuta T.
A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol.
PLoS One
2014
9(2):e88696
PubMed ID: 24533137
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SDS_DAP(hESM)_Japanese.pdf