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Cell No. : Cell Name
HES0001 : KhES-1  update : 2021/11/18
CommentHuman embryonic stem cell line. Please follow the guidelines of the Japanese Government for the procedure.
Comment from the depositor
Terms and conditions1) Please access "Human ES cell project information disclosure page". 2) Please read carefully the Japanese governmental guidelines on the utilization of human embryonic stem (ES) cells .
Order Form Order Form(C-0061.pdf)   Approval Form(C-0006.pdf)   MTA(C-0062.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nakatsuji, Norio
Originator Nakatsuji, Norio
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B231
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 5ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/3-4 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 3 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium DAP213 : Medium + 2M DMSO + 1M Acetamide + 3M Propyleneglycol
Freezing method Vitrification
Mycoplasma (-)
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 7
User's Publication 11

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5705  Otsuji TG, Kurose Y, Suemori H, Tada M, Nakatsuji N.  Dynamic link between histone H3 acetylation and an increase in the functional characteristics of human ESC/iPSC-derived cardiomyocytes.  PLoS One  2012  7(9):e45010  PubMed ID: 22984602
3678  Sakurai K, Shimoji M, Tahimic CG, Aiba K, Kawase E, Hasegawa K, Amagai Y, Suemori H, Nakatsuji N.  Efficient integration of transgenes into a defined locus in human embryonic stem cells.  Nucleic Acids Res  2010  38(7):e96  PubMed ID: 20071742
3682  Hasegawa K, Cowan AB, Nakatsuji N, Suemori H.  Efficient multicistronic expression of a transgene in human embryonic stem cells.  Stem Cells  2007  25(7):1707-12  PubMed ID: 17395772
3679  Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.  Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.  Biochem Biophys Res Commun  2006  345(3):926-32  PubMed ID: 16707099
3680  Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H.  A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.  Stem Cells  2006  24(12):2649-60  PubMed ID: 16931777
3677  Nakatsuji N.  Establishment and manipulation of monkey and human embryonic stem cell lines for biomedical research.  Ernst Schering Res Found Workshop  2005  54:15-26.  PubMed ID: 16080284
10895  Tsuyoshi Fujioka, Kentaro Yasuchika, Yukio Nakamura, Norio Nakatsuji, Hirofumi Suemori  A Simple and Efficient Cryopreservation Method for Primate Embryonic Stem Cells  Int J Dev Biol  2004  48(10):1149-54  PubMed ID: 15602701

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User's Publication
20662  Hayashi R, Okubo T, Kudo Y, Ishikawa Y, Imaizumi T, Suzuki K, Shibata S, Katayama T, Park SJ, Young RD, Quantock AJ, Nishida K.  Generation of 3D lacrimal gland organoids from human pluripotent stem cells.  Nature  2022    PubMed ID: 35444274
7003  Yamamoto T, Takenaka C, Yoda Y, Oshima Y, Kagawa K, Miyajima H, Sasaki T, Kawamata S.  Differentiation potential of Pluripotent Stem Cells correlates to the level of CHD7.  Sci Rep  2018  8:241  PubMed ID: 29321579
4274  Kano M, Suga H, Kasai T, Ozone C, Arima H.  Functional Pituitary Tissue Generation from Human Embryonic Stem Cells.  Curr Protoc Neurosci  2018    PubMed ID: 30040229
7009  Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe SI, Nishina S, Azuma N.  Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.  Sci Rep  2017  7:16757  PubMed ID: 29196712
13931  Ota A, Matsumura K, Lee JJ, Sumi S, Hyon SH.  StemCell Keep™ Is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification  Cell Transplant  2017  26(5):773-787  PubMed ID: 27503846
7315  Imaizumi K, Iha M, Nishishita N, Kawamata S, Nishikawa S, Akuta T.  A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.  Methods Mol. Biol.  2016  1341:15-24  PubMed ID: 26069023
9526  Ozone C, Suga H, Eiraku M, Kadoshima T, Yonemura S, Takata N, Oiso Y, Tsuji T, Sasai Y.  Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.  Nat Commun  2016  7:10351  PubMed ID: 26762480
9553  Sakaguchi H, Kadoshima T, Soen M, Narii N, Ishida Y, Ohgushi M, Takahashi J, Eiraku M, Sasai Y.  Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue.  Nat Commun  2015  6:8896  PubMed ID: 26573335
5749  Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, Ohshima Y, Kagawa K, Sasaki T, Kawamata S.  Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.  PLoS One  2015  10(6):e0129855  PubMed ID: 26115194
11943  Naoki Nishishita, Marie Muramatsu, Shin Kawamata  An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions  Am J Stem Cells  2015  4(1):38-49  PubMed ID: 25973330
5725  Imaizumi K, Nishishita N, Muramatsu M, Yamamoto T, Takenaka C, Kawamata S, Kobayashi K, Nishikawa S, Akuta T.  A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol.  PLoS One  2014  9(2):e88696  PubMed ID: 24533137

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