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Cell No. : Cell Name
GMC0035 : B0026 update : 2026/06/19 |
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| Comment | Human B cell line derived from Werner syndrome patient and transformed by Epstein-Barr Virus. The same patient as GMC0036. | |||
| Comment from the depositor | ||||
| Terms and conditions | A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0052.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). Regarding commercial use or use related to patent applications, please first contact the RIKEN BRC at cellbank.brc@riken.jp. | ||||
| Basic information | Depositor | Goto, Makoto | ||
| Originator | Danjoh, Inaho | |||
| Year of deposit | 2009 | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Race | Japanese | |||
| Gender | Female | |||
| Age at sampling | 37 years | |||
| Tissue | peripheral blood lymphocytes | |||
| Case history | Cataract,Characteristic skin,Bird face,Short stat | |||
| Genetics | Werner's syndrome | |||
| Classification | transformed | |||
| Year of origin | 2009 | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| SDS |
SDS_DMSO(Japanese) 
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| Cellosaurus(Expasy) | CVCL_T363 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method |
General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium | RPMI1640 + 20% FBS | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 4-6 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Virus (HIV) | Undetected | |||
| Virus (HTLV-1) | (-) | |||
| STR(human) | OK | |||
| Images | ||||
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| Reference information | Reference | 4 | ||
| User's Publication | 0 | |||
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