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Cell No. : Cell Name
GMC0032 : B0078  update : 2018/07/06
CommentHuman B cell line derived from Werner syndrome (questionable) patient and transformed by Epstein-Barr Virus.
Comment from the depositor
Terms and conditionsRequired written permission from the approver.
approver's address
Toin University Of Yokohama

1614 Kurogane-machi, Aoba-ku, Yokohama, Kanagawa, 225-8502 Japan
Dr.Goto, Makoto
Fax. +81-45-974-5193
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Basic information Depositor Goto, Makoto
Originator Danjoh, Inaho
Year of deposit 2009
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 59 years
Tissue peripheral blood lymphocytes
Case history Cataract,Flatfootedness,Mellitus
Genetics Werner's syndrome (questionable)
Classification transformed
Year of origin 2009
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 20% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
deposit info
lot info
Reference information Reference 1
User's Publication 0

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4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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