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Cell No. : Cell Name
GMC0025 : B0010  update : 2018/07/06
CommentHuman B cell line derived from Werner syndrome (questionable) patient and transformed by Epstein-Barr Virus. The same patient as GMC0027.
Comment from the depositor
Terms and conditionsRequired written permission from the approver.
Remarks
approver's address
×
English
Address
Toin University Of Yokohama

1614 Kurogane-machi, Aoba-ku, Yokohama, Kanagawa, 225-8502 Japan
Dr.Goto, Makoto
Fax. +81-45-974-5193
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Goto, Makoto
Originator Danjoh, Inaho
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 7 years
Tissue peripheral blood lymphocytes
Case history Cataract,Characteristic skin,Bird face,Short stat
Genetics Werner's syndrome (questionable)
Classification transformed
Year of origin 2008
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 20% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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