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Cell No. : Cell Name
GMC0024 : A0072  update : 2018/07/06
CommentHuman primary adherent cells derived from Werner syndrome (questionable) patient's skin. The same patient as GMC0020.
Comment from the depositor
Terms and conditionsRequired written permission from the approver.
Remarks
approver's address
×
English
Address
Toin University Of Yokohama

1614 Kurogane-machi, Aoba-ku, Yokohama, Kanagawa, 225-8502 Japan
Dr.Goto, Makoto
Fax. +81-45-974-5193
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Goto, Makoto
Originator Saijo, Kaoru
Year of deposit 2008
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 55 years
Tissue skin
Case history Cataract, Atherosclerosis, Hypertension
Genetics Werner's syndrome (questionable)
Classification normal
Year of origin 2008
Lifespan finite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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