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Cell No. : Cell Name
GMC0015 : B0042  update : 2024/01/17
CommentHuman B cell line derived from Werner syndrome (questionable) patient and transformed by Epstein-Barr Virus. The same patient as GMC0014.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
1-11-11-101 Kitazakae, Urayasy-shi, Chiba, 279-0002 Japan
Urayasu Rheumatology Clinic
E-mail. werner.goto@gmail.com
Dr.GOTO Makoto
Order Form Order Form(C-0052.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Goto, Makoto
Originator Danjoh, Inaho
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 53 years
Tissue peripheral blood lymphocytes
Genetics Werner's syndrome (questionable)
Classification transformed
Year of origin 2008
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T342
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 20% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014   DOI: 10.5582/irdr.2018.01092
919  Makoto Goto, Junji Chiba, Masaaki Matsuura, Sachiko Iwaki-Egawa, Yasuhiro Watanabe  Inflammageing assessed by MMP9 in normal Japanese individuals and the patients with Werner syndrome  Intractable Rare Dis Res  2016  5(2):103-8  PubMed ID: 27195193   DOI: 10.5582/irdr.2016.01028
918  M Goto, Y Ishikawa, M Sugimoto, Y Furuichi  Werner syndrome: a changing pattern of clinical manifestations in Japan (1917~2008)  Biosci Trends  2013  7(1):13-22  PubMed ID: 23524889  
920  M Goto, S Iwaki-Egawa, Y Watanabe  Ageing in Werner syndrome  Biosci Trends  2012  6(1):33-7  PubMed ID: 22426101   DOI: 10.5582/bst.2012.v6.1.33

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