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Cell No. : Cell Name
GMC0012 : A0031  update : 2022/12/20
CommentHuman primary adherent cells derived from Werner syndrome patient's skin. The same patient as GMC0005.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
1-11-11-101 Kitazakae, Urayasy-shi, Chiba, 279-0002 Japan
Urayasu Rheumatology Clinic
E-mail. werner.goto@gmail.com
Dr.GOTO Makoto
Order Form Order Form(C-0052.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Goto, Makoto
Originator Saijo, Kaoru
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 37 years
Tissue skin
Case history Cataract,Characteristic skin,Mellitus
Genetics Werner's syndrome
Classification normal
Year of origin 2008
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T338
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 5
User's Publication 1


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014   DOI: 10.5582/irdr.2018.01092
919  Makoto Goto, Junji Chiba, Masaaki Matsuura, Sachiko Iwaki-Egawa, Yasuhiro Watanabe  Inflammageing assessed by MMP9 in normal Japanese individuals and the patients with Werner syndrome  Intractable Rare Dis Res  2016  5(2):103-8  PubMed ID: 27195193   DOI: 10.5582/irdr.2016.01028
12214  Sadahira Y, Sugihara T, Fujiwara H, Nishimura H, Suetsugu Y, Takeshita M, Okamura S, Goto M.  WRN protein as a novel erythroblast immunohistochemical marker with applications for the diagnosis of Werner syndrome  Virchows Arch  2015  466(3):343-50  PubMed ID: 25503078   DOI: 10.1007/s00428-014-1703-6
918  M Goto, Y Ishikawa, M Sugimoto, Y Furuichi  Werner syndrome: a changing pattern of clinical manifestations in Japan (1917~2008)  Biosci Trends  2013  7(1):13-22  PubMed ID: 23524889  
920  M Goto, S Iwaki-Egawa, Y Watanabe  Ageing in Werner syndrome  Biosci Trends  2012  6(1):33-7  PubMed ID: 22426101   DOI: 10.5582/bst.2012.v6.1.33

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User's Publication
5455  Shimamoto A, Kagawa H, Zensho K, Sera Y, Kazuki Y, Osaki M, Oshimura M, Ishigaki Y, Hamasaki K, Kodama Y, Yuasa S, Fukuda K, Hirashima K, Seimiya H, Koyama H, Shimizu T, Takemoto M, Yokote K, Goto M, Tahara H.  Reprogramming suppresses premature senescence phenotypes of Werner syndrome cells and maintains chromosomal stability over long-term culture.  PLoS One.  2014  9(11):e112900  PubMed ID: 25390333   DOI: 10.1371/journal.pone.0112900


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