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Cell No. : Cell Name
GMC0010 : UN7RGB  update : 2021/05/20
CommentQuestionable Werner's syndrome-like. Life span in vitro was over 60 PDL.
Comment from the depositor
Terms and conditionsRequired written permission from the approver.
Remarks
approver's address
×
English
Address
1-11-11-101 Kitazakae, Urayasy-shi, Chiba, 279-0002 Japan
Urayasu Rheumatology Clinic
E-mail. werner.goto@gmail.com
Dr.Goto, Makoto
Order Form Order Form(C-0052.pdf)   Approval Form(C-0006.pdf)   MTA(C-0053.pdf)   MTA(C-0053p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Goto, Makoto
Originator Ohno, T.
Year of deposit 1993
Another name RCB0873
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 45 years
Tissue skin
Case history Werner syndrome-like, but not.
Classification normal
Lifespan finite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T332
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(2),45(1),46(45),57(1),92(1)
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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