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Cell No. : Cell Name
GMC0009 : WS6RGB  update : 2018/07/06
CommentWerner's syndrome fibroblast
Comment from the depositor
Terms and conditionsRequired written permission from the approver.
Remarks
approver's address
×
English
Address
Toin University Of Yokohama

1614 Kurogane-machi, Aoba-ku, Yokohama, Kanagawa, 225-8502 Japan
Dr.Goto, Makoto
Fax. +81-45-974-5193
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Goto, Makoto
Originator Ohno, Tadao
Year of deposit 1989
Another name RCB0387
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 52 years
Tissue skin
Genetics Werner's syndrome
Classification normal
Year of origin 1989
Lifespan finite
Crisis PDL 22 PDL
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 43-47(50) : 43(1),44(3),45(9),46(36),47(1)
Reference information Reference 1
User's Publication 1


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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User's Publication
2825  Zhang Z, Hirano K, Tsukamoto K, Ikegami C, Koseki M, Saijo K, Ohno T, Sakai N, Hiraoka H, Shimomura I, Yamashita S  Defective cholesterol efflux in Werner syndrome fibroblasts and its phenotypic correction by Cdc42, a RhoGTPase.  Exp Gerontol  2005  40:286-94  PubMed ID: 15820609



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